Ribotyping of Lactobacillus helveticus from the Koumiss
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00012625" target="_blank" >RIV/00216224:14310/05:00012625 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Ribotyping of Lactobacillus helveticus from the Koumiss
Popis výsledku v původním jazyce
The lactic acid bacteria (LAB) are very important components of the formation of milk products. Lactobacillus helveticus, a homofermentative thermophilic LAB largely presented in cheeses, has been found to be the prevalent species in natural koumiss. A collection of six L. helveticus strains supplemented with reference cultures was studied for its phenotypic characters, as well as for genotyping. Biotyping (API 50CH kit, tube tests) confirmed that isolates belong to the genus Lactobacillus, but it was not suitable for identification to the species level. A phenotypic heterogeneity among L. helveticus isolates was observed, mainly in acid formation from sugars. Ribotyping (EcoRI, DNA probe complementary to 16S and 23S rRNA) yielded a variety of restriction patterns and allowed good strain differentiation. Obtained ribotypes of analyzed isolates gave unique band patterns with no relationship to the selected reference cultures.
Název v anglickém jazyce
Ribotyping of Lactobacillus helveticus from the Koumiss
Popis výsledku anglicky
The lactic acid bacteria (LAB) are very important components of the formation of milk products. Lactobacillus helveticus, a homofermentative thermophilic LAB largely presented in cheeses, has been found to be the prevalent species in natural koumiss. A collection of six L. helveticus strains supplemented with reference cultures was studied for its phenotypic characters, as well as for genotyping. Biotyping (API 50CH kit, tube tests) confirmed that isolates belong to the genus Lactobacillus, but it was not suitable for identification to the species level. A phenotypic heterogeneity among L. helveticus isolates was observed, mainly in acid formation from sugars. Ribotyping (EcoRI, DNA probe complementary to 16S and 23S rRNA) yielded a variety of restriction patterns and allowed good strain differentiation. Obtained ribotypes of analyzed isolates gave unique band patterns with no relationship to the selected reference cultures.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GP204%2F02%2FD099" target="_blank" >GP204/02/D099: Knihovna ribotypizačních profilů typových kultur bakterií</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2005
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů