Molecular characterization of brewing and wine yeast strains in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00069170" target="_blank" >RIV/00216224:14310/13:00069170 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60193697:_____/13:#0000894

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/yea.v30.S1/issuetoc" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/yea.v30.S1/issuetoc</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular characterization of brewing and wine yeast strains in the Czech Republic

Popis výsledku v původním jazyce

The hybrid origin of lager yeasts causes problems in determination of the technologically diverse species S. pastorianus and S. cerevisiae. Wine and brewing yeast S. cerevisiae could be differentiated on the DNA level. In our study we chose FLO and HIS4gene, the regions ITS, RRL2 and YBR033w and mtDNA as target sequences. A group of 28 brewing, wine and type yeast strains was analyzed by PCR and RFLP techniques. Detection of flocculation genes did not document any species-specific representation in anyof the strain under study. Gene LgFLO, considered as typical for lager yeast, was detected also in ale yeast. Other analyses led to an identical species classification only in 7 yeast strains. Using digestion of the ITS region, 26 strains, including allof lager yeast were identified as S. cerevisiae; this region is thus unsuitable for identification of industrial yeast strains. Identification of ale and lager yeast should partially reflect the maximum temperature of growth.

Název v anglickém jazyce

Molecular characterization of brewing and wine yeast strains in the Czech Republic

Popis výsledku anglicky

The hybrid origin of lager yeasts causes problems in determination of the technologically diverse species S. pastorianus and S. cerevisiae. Wine and brewing yeast S. cerevisiae could be differentiated on the DNA level. In our study we chose FLO and HIS4gene, the regions ITS, RRL2 and YBR033w and mtDNA as target sequences. A group of 28 brewing, wine and type yeast strains was analyzed by PCR and RFLP techniques. Detection of flocculation genes did not document any species-specific representation in anyof the strain under study. Gene LgFLO, considered as typical for lager yeast, was detected also in ale yeast. Other analyses led to an identical species classification only in 7 yeast strains. Using digestion of the ITS region, 26 strains, including allof lager yeast were identified as S. cerevisiae; this region is thus unsuitable for identification of industrial yeast strains. Identification of ale and lager yeast should partially reflect the maximum temperature of growth.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů