Molecular characterization of brewing and wine yeast strains in the Czech Republic
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00069170" target="_blank" >RIV/00216224:14310/13:00069170 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60193697:_____/13:#0000894
Výsledek na webu
<a href="http://onlinelibrary.wiley.com/doi/10.1002/yea.v30.S1/issuetoc" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/yea.v30.S1/issuetoc</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Molecular characterization of brewing and wine yeast strains in the Czech Republic
Popis výsledku v původním jazyce
The hybrid origin of lager yeasts causes problems in determination of the technologically diverse species S. pastorianus and S. cerevisiae. Wine and brewing yeast S. cerevisiae could be differentiated on the DNA level. In our study we chose FLO and HIS4gene, the regions ITS, RRL2 and YBR033w and mtDNA as target sequences. A group of 28 brewing, wine and type yeast strains was analyzed by PCR and RFLP techniques. Detection of flocculation genes did not document any species-specific representation in anyof the strain under study. Gene LgFLO, considered as typical for lager yeast, was detected also in ale yeast. Other analyses led to an identical species classification only in 7 yeast strains. Using digestion of the ITS region, 26 strains, including allof lager yeast were identified as S. cerevisiae; this region is thus unsuitable for identification of industrial yeast strains. Identification of ale and lager yeast should partially reflect the maximum temperature of growth.
Název v anglickém jazyce
Molecular characterization of brewing and wine yeast strains in the Czech Republic
Popis výsledku anglicky
The hybrid origin of lager yeasts causes problems in determination of the technologically diverse species S. pastorianus and S. cerevisiae. Wine and brewing yeast S. cerevisiae could be differentiated on the DNA level. In our study we chose FLO and HIS4gene, the regions ITS, RRL2 and YBR033w and mtDNA as target sequences. A group of 28 brewing, wine and type yeast strains was analyzed by PCR and RFLP techniques. Detection of flocculation genes did not document any species-specific representation in anyof the strain under study. Gene LgFLO, considered as typical for lager yeast, was detected also in ale yeast. Other analyses led to an identical species classification only in 7 yeast strains. Using digestion of the ITS region, 26 strains, including allof lager yeast were identified as S. cerevisiae; this region is thus unsuitable for identification of industrial yeast strains. Identification of ale and lager yeast should partially reflect the maximum temperature of growth.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů