PCR and restriction analysis of brewing and wine yeasts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00070098" target="_blank" >RIV/00216224:14310/13:00070098 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

PCR and restriction analysis of brewing and wine yeasts

Popis výsledku v původním jazyce

Bottom fermenting brewing yeast (S. pastorianus) is a hybrid polyploid microorganism, most often triploid, tetraploid or aneuploid. The hybrid origin of brewing yeast causes problems in identification, including determination of the technologically diverse species S. pastorianus and S. cerevisiae. Wine yeast are mostly diploid and belong to the species S. cerevisiae and S. bayanus. In our study a group of at least 40 brewing, 8 wine and 3 type yeast strains was analyzed by PCR and RFLP techniques. Restriction of mtDNA with 4 different enzymes resulted in 9-12 restriction patterns, an identical pattern was shared by 4 out of 10 ale and lager strain. Wine yeast exhibited a restriction pattern different from that of lager strains. According to the RAPD method, wine and top fermenting strains are clearly separated from lager strains except for 4 top fermenting strains.

Název v anglickém jazyce

PCR and restriction analysis of brewing and wine yeasts

Popis výsledku anglicky

Bottom fermenting brewing yeast (S. pastorianus) is a hybrid polyploid microorganism, most often triploid, tetraploid or aneuploid. The hybrid origin of brewing yeast causes problems in identification, including determination of the technologically diverse species S. pastorianus and S. cerevisiae. Wine yeast are mostly diploid and belong to the species S. cerevisiae and S. bayanus. In our study a group of at least 40 brewing, 8 wine and 3 type yeast strains was analyzed by PCR and RFLP techniques. Restriction of mtDNA with 4 different enzymes resulted in 9-12 restriction patterns, an identical pattern was shared by 4 out of 10 ale and lager strain. Wine yeast exhibited a restriction pattern different from that of lager strains. According to the RAPD method, wine and top fermenting strains are clearly separated from lager strains except for 4 top fermenting strains.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů