Monitoring of HPLC profiles of selected polyphenolic compounds in sea buckthorn (Hippophaë rhamnoides L.) plant parts during annual growth cycle and estimation of their antioxidant potential

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00075589" target="_blank" >RIV/00216224:14310/14:00075589 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28110/14:43871780

Výsledek na webu

<a href="http://link.springer.com/article/10.2478%2Fs11532-014-0562-y" target="_blank" >http://link.springer.com/article/10.2478%2Fs11532-014-0562-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11532-014-0562-y" target="_blank" >10.2478/s11532-014-0562-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of HPLC profiles of selected polyphenolic compounds in sea buckthorn (Hippophaë rhamnoides L.) plant parts during annual growth cycle and estimation of their antioxidant potential

Popis výsledku v původním jazyce

Presented work summarizes the data about polyphenolic profiles in various plant parts (leaves, shoots, berries) of sea buckthorn (Hippophaë rhamnoides L.) during the annual growth cycle. A reversed-phase high performance liquid chromatography method (RP-HPLC) coupled with diode-array detection (DAD) was optimized for determination of catechin, epicatechin, gallic acid, p-coumaric acid, caffeic acid, ferulic acid, rutin (quercetin 3-rutinoside) and quercitrin (quercetin 3-rhamnoside). The content of these polyphenolic compounds was monitored in extracts of sea buckthorn plant samples from April to October. The total antioxidant activity was determined using scavenging of 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonate) cation radical (ABTSx+) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPHx). The total content of polyphenols was estimated by conventional spectrophotometric method using Folin-Ciocalteu reagent.

Název v anglickém jazyce

Monitoring of HPLC profiles of selected polyphenolic compounds in sea buckthorn (Hippophaë rhamnoides L.) plant parts during annual growth cycle and estimation of their antioxidant potential

Popis výsledku anglicky

Presented work summarizes the data about polyphenolic profiles in various plant parts (leaves, shoots, berries) of sea buckthorn (Hippophaë rhamnoides L.) during the annual growth cycle. A reversed-phase high performance liquid chromatography method (RP-HPLC) coupled with diode-array detection (DAD) was optimized for determination of catechin, epicatechin, gallic acid, p-coumaric acid, caffeic acid, ferulic acid, rutin (quercetin 3-rutinoside) and quercitrin (quercetin 3-rhamnoside). The content of these polyphenolic compounds was monitored in extracts of sea buckthorn plant samples from April to October. The total antioxidant activity was determined using scavenging of 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonate) cation radical (ABTSx+) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPHx). The total content of polyphenols was estimated by conventional spectrophotometric method using Folin-Ciocalteu reagent.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Central European Journal of Chemistry

ISSN

1895-1066

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

10

Strana od-do

1152-1161

Kód UT WoS článku

000336308900005

EID výsledku v databázi Scopus

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