Comparison of DNA-based techniques for differentiation of production strains of ale and lager brewing yeast

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00089649" target="_blank" >RIV/00216224:14310/16:00089649 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/jam.13116" target="_blank" >http://dx.doi.org/10.1111/jam.13116</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jam.13116" target="_blank" >10.1111/jam.13116</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of DNA-based techniques for differentiation of production strains of ale and lager brewing yeast

Popis výsledku v původním jazyce

Brewing yeasts are classified into two species—Saccharomyces pastorianus and Saccharomyces cerevisiae. Most of the brewing yeast strains are natural interspecies hybrids typically polyploids and their identification is thus often difficult giving heterogenous results according to the method used. We performed genetic characterization of a set of the brewing yeast strains coming from several yeast culture collections by combination of various DNA-based techniques. The aim of this study was to select a method for species-specific identification of yeast and discrimination of yeast strains according to their technological classification. PCR-RFLP of HIS4 gene and RAPD-PCR of genomic DNA are reliable and suitable methods for fast identification of yeast strains. RAPD-PCR with primer 21 is a fast and reliable method applicable for differentiation of brewing yeasts with only 35% similarity of fingerprint profile between the two main technological groups (ale and lager) of brewing strains.

Název v anglickém jazyce

Comparison of DNA-based techniques for differentiation of production strains of ale and lager brewing yeast

Popis výsledku anglicky

Brewing yeasts are classified into two species—Saccharomyces pastorianus and Saccharomyces cerevisiae. Most of the brewing yeast strains are natural interspecies hybrids typically polyploids and their identification is thus often difficult giving heterogenous results according to the method used. We performed genetic characterization of a set of the brewing yeast strains coming from several yeast culture collections by combination of various DNA-based techniques. The aim of this study was to select a method for species-specific identification of yeast and discrimination of yeast strains according to their technological classification. PCR-RFLP of HIS4 gene and RAPD-PCR of genomic DNA are reliable and suitable methods for fast identification of yeast strains. RAPD-PCR with primer 21 is a fast and reliable method applicable for differentiation of brewing yeasts with only 35% similarity of fingerprint profile between the two main technological groups (ale and lager) of brewing strains.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Microbiology

ISSN

1364-5072

e-ISSN

—

Svazek periodika

120

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

1561-1573

Kód UT WoS článku

000379534800011

EID výsledku v databázi Scopus

—