Topography of telomeres and structure of chromosome territories in human lymphocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F03%3A00008239" target="_blank" >RIV/00216224:14330/03:00008239 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Topography of telomeres and structure of chromosome territories in human lymphocytes

Popis výsledku v původním jazyce

Nuclear and territorial positioning of p- and q- telomeres and centromeres of chromosomes 3, 8, 9, 13 and 19 were studied by repeated FISH, high-resolution cytometry and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the opposite side of the territories as compared with the centromeres for all chromosome territories investigated. Mutual distances between telomeres of submetacentric chromosomes were very short, usually shorter than centromere-to-telomere distances, which means that the chromosome territory is non-randomly folded. Telomeres are in average much nearer to the center of the cell nucleus than centromeres; q-telomeres were found in average more centrally localized as comparedwith p-telomeres. Consequently, we directly showed that chromosome territories in the cell nucleus are (i) polar and (ii) partially oriented in cell nuclei. The distributions of genetic elements relative to chromosome territories (territo

Název v anglickém jazyce

Topography of telomeres and structure of chromosome territories in human lymphocytes

Popis výsledku anglicky

Nuclear and territorial positioning of p- and q- telomeres and centromeres of chromosomes 3, 8, 9, 13 and 19 were studied by repeated FISH, high-resolution cytometry and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the opposite side of the territories as compared with the centromeres for all chromosome territories investigated. Mutual distances between telomeres of submetacentric chromosomes were very short, usually shorter than centromere-to-telomere distances, which means that the chromosome territory is non-randomly folded. Telomeres are in average much nearer to the center of the cell nucleus than centromeres; q-telomeres were found in average more centrally localized as comparedwith p-telomeres. Consequently, we directly showed that chromosome territories in the cell nucleus are (i) polar and (ii) partially oriented in cell nuclei. The distributions of genetic elements relative to chromosome territories (territo

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Biophysics of the Genome

ISBN

80-210-3226-X

ISSN

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e-ISSN

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Počet stran výsledku

11

Strana od-do

1-11

Název nakladatele

Masaryk University

Místo vydání

Brno

Místo konání akce

Hlovovec, Czech Republic

Datum konání akce

2. 4. 2003

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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