Topographic characterization of telomeres in healthy and various tumor cell lines determined in situ and in vivo
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F04%3A00009715" target="_blank" >RIV/00216224:14330/04:00009715 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Topographic characterization of telomeres in healthy and various tumor cell lines determined in situ and in vivo
Popis výsledku v původním jazyce
Nuclear and territorial positioning of p- and q- telomeres and centromeres of chromosomes 3, 8, 9, 13 and 19 were studied using repeated fluorescence in situ hybridization, high-resolution image cytometry and three-dimensional image analysis of human blood lymphocytes before and after stimulation (Amrichová et al., 2003). Our results suggest that in both G0- and stimulated lymphocytes, centromeres of HSA 3, 8, 9, and 13 are predominantly localized at the periphery of the cell nucleus; only centromere ofHSA 19 in G0-lymphocytes is localized more centrally. Both p- and q-telomeres mostly adopt positions in the nuclear interior; q-telomeres are usually nearer to the center of the cell nucleus as compared to p-telomeres. Using repeated hybridization, tethering between telomeres of heterologous chromosomes 8, 9, and 19 was investigated. An attempt was made to find which particular telomeres are involved in telomere associations and whether any associations can be found also between homolog
Název v anglickém jazyce
Topographic characterization of telomeres in healthy and various tumor cell lines determined in situ and in vivo
Popis výsledku anglicky
Nuclear and territorial positioning of p- and q- telomeres and centromeres of chromosomes 3, 8, 9, 13 and 19 were studied using repeated fluorescence in situ hybridization, high-resolution image cytometry and three-dimensional image analysis of human blood lymphocytes before and after stimulation (Amrichová et al., 2003). Our results suggest that in both G0- and stimulated lymphocytes, centromeres of HSA 3, 8, 9, and 13 are predominantly localized at the periphery of the cell nucleus; only centromere ofHSA 19 in G0-lymphocytes is localized more centrally. Both p- and q-telomeres mostly adopt positions in the nuclear interior; q-telomeres are usually nearer to the center of the cell nucleus as compared to p-telomeres. Using repeated hybridization, tethering between telomeres of heterologous chromosomes 8, 9, and 19 was investigated. An attempt was made to find which particular telomeres are involved in telomere associations and whether any associations can be found also between homolog
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2004
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů