Nuclear and territorial topography of chromosome telomeres in human lymphocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F03%3A00008632" target="_blank" >RIV/00216224:14330/03:00008632 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/03:17033135 RIV/00216224:14330/03:00008546 RIV/00216224:14330/03:00008551 RIV/00216224:14330/03:00008545

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Nuclear and territorial topography of chromosome telomeres in human lymphocytes

Popis výsledku v původním jazyce

Nuclear and territorial positioning of p- and q- telomeres and centromeres of chromosomes 3, 8, 9, 13 and 19 were studied by repeated FISH, high-resolution cytometry and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the opposite side of the territories as compared with the centromeres for all chromosome territories investigated. Mutual distances between telomeres of submetacentric chromosomes were very short, usually shorter than centromere-to-telomere distances, which means that the chromosome territory is non-randomly folded. Telomeres are in average much nearer to the center of the cell nucleus than centromeres; q-telomeres were found in average more centrally localized as comparedwith p-telomeres. Consequently, we directly showed that chromosome territories in the cell nucleus are (i) polar and (ii) partially oriented in cell nuclei. The distributions of genetic elements relative to chromosome territories (territo

Název v anglickém jazyce

Nuclear and territorial topography of chromosome telomeres in human lymphocytes

Popis výsledku anglicky

Nuclear and territorial positioning of p- and q- telomeres and centromeres of chromosomes 3, 8, 9, 13 and 19 were studied by repeated FISH, high-resolution cytometry and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the opposite side of the territories as compared with the centromeres for all chromosome territories investigated. Mutual distances between telomeres of submetacentric chromosomes were very short, usually shorter than centromere-to-telomere distances, which means that the chromosome territory is non-randomly folded. Telomeres are in average much nearer to the center of the cell nucleus than centromeres; q-telomeres were found in average more centrally localized as comparedwith p-telomeres. Consequently, we directly showed that chromosome territories in the cell nucleus are (i) polar and (ii) partially oriented in cell nuclei. The distributions of genetic elements relative to chromosome territories (territo

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental Cell Research

ISSN

0014-4827

e-ISSN

—

Svazek periodika

289

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

11-26

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—