Automated confocal microscopy: The way of achieving both quality and quantity in 3D image cytometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F04%3A00028399" target="_blank" >RIV/00216224:14330/04:00028399 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Automated confocal microscopy: The way of achieving both quality and quantity in 3D image cytometry

Popis výsledku v původním jazyce

Image cytometry is mostly associated with high quality but low quantity, whereas flow cytometry is associated with high quantity but low quality. Achieving both quality and quantity in cytometry is possible in the case of automated confocal microscopy systems. We have shown that the automation of image acquisition and image processing in confocal microscopy is possible for specific tasks such as 2D and 3D FISH imaging. This paper is focused on practical problems and solutions that we have encountered during our research. We believe that our experience might be helpful to all those who decide to automate their light microscopy systems for cell studies, i.e. for image cytometry purposes.

Název v anglickém jazyce

Automated confocal microscopy: The way of achieving both quality and quantity in 3D image cytometry

Popis výsledku anglicky

Image cytometry is mostly associated with high quality but low quantity, whereas flow cytometry is associated with high quantity but low quality. Achieving both quality and quantity in cytometry is possible in the case of automated confocal microscopy systems. We have shown that the automation of image acquisition and image processing in confocal microscopy is possible for specific tasks such as 2D and 3D FISH imaging. This paper is focused on practical problems and solutions that we have encountered during our research. We believe that our experience might be helpful to all those who decide to automate their light microscopy systems for cell studies, i.e. for image cytometry purposes.

Druh

O - Ostatní výsledky

CEP obor

JD - Využití počítačů, robotika a její aplikace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů