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Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00121457" target="_blank" >RIV/00216224:14740/20:00121457 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/62156489:43210/20:43917906 RIV/00216305:26620/20:PU136753

  • Výsledek na webu

    <a href="https://www.sciencedirect.com/science/article/pii/S0925400520304913?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0925400520304913?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.snb.2020.128141" target="_blank" >10.1016/j.snb.2020.128141</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

  • Popis výsledku v původním jazyce

    A simple and reproducible electroanalytical sensor using antimony trioxide modified-carbon paste electrode (Sb2O3-CPE) as sensing element was employed for the first time for simultaneous determination of glutathione (GSH) and glutathione disulphide (GSSG). The electrochemical response of Sb2O3-CPE was characterized by cyclic voltammetry and square-wave voltammetry. Scanning electron microscopy (SEM), scanning electrochemical microscopy (SECM) and Raman mapping were used to characterize the morphology and surface modification. A significant enhancement in the peak current response of GSH and GSSG accompanied with a good separation of the peaks were obtained at the modified electrode compared to the bare CPE. GSH and GSSG exhibited well-defined oxidation peaks at +1.08 V and +1.36 V (vs. Ag/AgCl, 3 mol/L) in Britton-Robinson buffer at pH 6.0. Parameters such as used supporting electrolyte and its pH value, amplitude and frequency were optimized for square-wave voltammetric determination. Under optimal conditions, voltammetric responses were linear in the concentration range of 2-200 mu mol/L for GSH and GSSG. The limits of detection were 0.34 mu mol/L and 0.10 mu mol/L for GSH and GSSG, respectively. Moreover, the proposed sensor also showed good reproducibility, stability and selectivity. Finally, the proposed sensor was applied for the simultaneous determination of GSH and GSSG in urine samples with satisfactory results.

  • Název v anglickém jazyce

    Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

  • Popis výsledku anglicky

    A simple and reproducible electroanalytical sensor using antimony trioxide modified-carbon paste electrode (Sb2O3-CPE) as sensing element was employed for the first time for simultaneous determination of glutathione (GSH) and glutathione disulphide (GSSG). The electrochemical response of Sb2O3-CPE was characterized by cyclic voltammetry and square-wave voltammetry. Scanning electron microscopy (SEM), scanning electrochemical microscopy (SECM) and Raman mapping were used to characterize the morphology and surface modification. A significant enhancement in the peak current response of GSH and GSSG accompanied with a good separation of the peaks were obtained at the modified electrode compared to the bare CPE. GSH and GSSG exhibited well-defined oxidation peaks at +1.08 V and +1.36 V (vs. Ag/AgCl, 3 mol/L) in Britton-Robinson buffer at pH 6.0. Parameters such as used supporting electrolyte and its pH value, amplitude and frequency were optimized for square-wave voltammetric determination. Under optimal conditions, voltammetric responses were linear in the concentration range of 2-200 mu mol/L for GSH and GSSG. The limits of detection were 0.34 mu mol/L and 0.10 mu mol/L for GSH and GSSG, respectively. Moreover, the proposed sensor also showed good reproducibility, stability and selectivity. Finally, the proposed sensor was applied for the simultaneous determination of GSH and GSSG in urine samples with satisfactory results.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Návaznosti výsledku

  • Projekt

  • Návaznosti

Ostatní

  • Rok uplatnění

    2020

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Sensors and Actuators B: Chemical

  • ISSN

    0925-4005

  • e-ISSN

  • Svazek periodika

    318

  • Číslo periodika v rámci svazku

    SEP

  • Stát vydavatele periodika

    CH - Švýcarská konfederace

  • Počet stran výsledku

    12

  • Strana od-do

    128141

  • Kód UT WoS článku

    000539189500001

  • EID výsledku v databázi Scopus

    2-s2.0-85084634181