Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00121457" target="_blank" >RIV/00216224:14740/20:00121457 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43210/20:43917906 RIV/00216305:26620/20:PU136753
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0925400520304913?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0925400520304913?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.snb.2020.128141" target="_blank" >10.1016/j.snb.2020.128141</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode
Popis výsledku v původním jazyce
A simple and reproducible electroanalytical sensor using antimony trioxide modified-carbon paste electrode (Sb2O3-CPE) as sensing element was employed for the first time for simultaneous determination of glutathione (GSH) and glutathione disulphide (GSSG). The electrochemical response of Sb2O3-CPE was characterized by cyclic voltammetry and square-wave voltammetry. Scanning electron microscopy (SEM), scanning electrochemical microscopy (SECM) and Raman mapping were used to characterize the morphology and surface modification. A significant enhancement in the peak current response of GSH and GSSG accompanied with a good separation of the peaks were obtained at the modified electrode compared to the bare CPE. GSH and GSSG exhibited well-defined oxidation peaks at +1.08 V and +1.36 V (vs. Ag/AgCl, 3 mol/L) in Britton-Robinson buffer at pH 6.0. Parameters such as used supporting electrolyte and its pH value, amplitude and frequency were optimized for square-wave voltammetric determination. Under optimal conditions, voltammetric responses were linear in the concentration range of 2-200 mu mol/L for GSH and GSSG. The limits of detection were 0.34 mu mol/L and 0.10 mu mol/L for GSH and GSSG, respectively. Moreover, the proposed sensor also showed good reproducibility, stability and selectivity. Finally, the proposed sensor was applied for the simultaneous determination of GSH and GSSG in urine samples with satisfactory results.
Název v anglickém jazyce
Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode
Popis výsledku anglicky
A simple and reproducible electroanalytical sensor using antimony trioxide modified-carbon paste electrode (Sb2O3-CPE) as sensing element was employed for the first time for simultaneous determination of glutathione (GSH) and glutathione disulphide (GSSG). The electrochemical response of Sb2O3-CPE was characterized by cyclic voltammetry and square-wave voltammetry. Scanning electron microscopy (SEM), scanning electrochemical microscopy (SECM) and Raman mapping were used to characterize the morphology and surface modification. A significant enhancement in the peak current response of GSH and GSSG accompanied with a good separation of the peaks were obtained at the modified electrode compared to the bare CPE. GSH and GSSG exhibited well-defined oxidation peaks at +1.08 V and +1.36 V (vs. Ag/AgCl, 3 mol/L) in Britton-Robinson buffer at pH 6.0. Parameters such as used supporting electrolyte and its pH value, amplitude and frequency were optimized for square-wave voltammetric determination. Under optimal conditions, voltammetric responses were linear in the concentration range of 2-200 mu mol/L for GSH and GSSG. The limits of detection were 0.34 mu mol/L and 0.10 mu mol/L for GSH and GSSG, respectively. Moreover, the proposed sensor also showed good reproducibility, stability and selectivity. Finally, the proposed sensor was applied for the simultaneous determination of GSH and GSSG in urine samples with satisfactory results.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Návaznosti výsledku
Projekt
—
Návaznosti
—
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Sensors and Actuators B: Chemical
ISSN
0925-4005
e-ISSN
—
Svazek periodika
318
Číslo periodika v rámci svazku
SEP
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
12
Strana od-do
128141
Kód UT WoS článku
000539189500001
EID výsledku v databázi Scopus
2-s2.0-85084634181