Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917906" target="_blank" >RIV/62156489:43210/20:43917906 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/20:PU136753 RIV/00216224:14740/20:00121457

Výsledek na webu

<a href="https://doi.org/10.1016/j.snb.2020.128141" target="_blank" >https://doi.org/10.1016/j.snb.2020.128141</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2020.128141" target="_blank" >10.1016/j.snb.2020.128141</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

Popis výsledku v původním jazyce

A simple and reproducible electroanalytical sensor using antimony trioxide modified-carbon paste electrode (Sb2O3-CPE) as sensing element was employed for the first time for simultaneous determination of glutathione (GSH) and glutathione disulphide (GSSG). The electrochemical response of Sb2O3-CPE was characterized by cyclic voltammetry and square-wave voltammetry. Scanning electron microscopy (SEM), scanning electrochemical microscopy (SECM) and Raman mapping were used to characterize the morphology and surface modification. A significant enhancement in the peak current response of GSH and GSSG accompanied with a good separation of the peaks were obtained at the modified electrode compared to the bare CPE. GSH and GSSG exhibited well-defined oxidation peaks at +1.08 V and +1.36 V (vs. Ag/AgCl, 3 mol/L) in Britton-Robinson buffer at pH 6.0. Parameters such as used supporting electrolyte and its pH value, amplitude and frequency were optimized for square-wave voltammetric determination. Under optimal conditions, voltammetric responses were linear in the concentration range of 2-200 μmol/L for GSH and GSSG. The limits of detection were 0.34 μmol/L and 0.10 μmol/L for GSH and GSSG, respectively. Moreover, the proposed sensor also showed good reproducibility, stability and selectivity. Finally, the proposed sensor was applied for the simultaneous determination of GSH and GSSG in urine samples with satisfactory results.

Název v anglickém jazyce

Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

Popis výsledku anglicky

A simple and reproducible electroanalytical sensor using antimony trioxide modified-carbon paste electrode (Sb2O3-CPE) as sensing element was employed for the first time for simultaneous determination of glutathione (GSH) and glutathione disulphide (GSSG). The electrochemical response of Sb2O3-CPE was characterized by cyclic voltammetry and square-wave voltammetry. Scanning electron microscopy (SEM), scanning electrochemical microscopy (SECM) and Raman mapping were used to characterize the morphology and surface modification. A significant enhancement in the peak current response of GSH and GSSG accompanied with a good separation of the peaks were obtained at the modified electrode compared to the bare CPE. GSH and GSSG exhibited well-defined oxidation peaks at +1.08 V and +1.36 V (vs. Ag/AgCl, 3 mol/L) in Britton-Robinson buffer at pH 6.0. Parameters such as used supporting electrolyte and its pH value, amplitude and frequency were optimized for square-wave voltammetric determination. Under optimal conditions, voltammetric responses were linear in the concentration range of 2-200 μmol/L for GSH and GSSG. The limits of detection were 0.34 μmol/L and 0.10 μmol/L for GSH and GSSG, respectively. Moreover, the proposed sensor also showed good reproducibility, stability and selectivity. Finally, the proposed sensor was applied for the simultaneous determination of GSH and GSSG in urine samples with satisfactory results.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators B. Chemical

ISSN

0925-4005

e-ISSN

—

Svazek periodika

318

Číslo periodika v rámci svazku

1 September

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

128141

Kód UT WoS článku

000539189500001

EID výsledku v databázi Scopus

2-s2.0-85084634181