Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from Meat-Processing Plants by a Culture Technique and Detection by PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F03%3A00000614" target="_blank" >RIV/00216275:25310/03:00000614 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216275:25310/03:00006599

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from Meat-Processing Plants by a Culture Technique and Detection by PCR

Popis výsledku v původním jazyce

A pilot survey of sources of contamination with arcobacters, which represent a potential risk for humans, is described. A wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.), both from a retail level and domestic farming. Sanitary practices in abattoirs and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The applied method was based on a selective enrichment to isolate suspect strains,in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical for the genus Arcobacter, and a product of 686 bp, specific for A. butzleri. On the whole, a total number of 198 samples was tested, of that 33 (16.7%) were found to

Název v anglickém jazyce

Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from Meat-Processing Plants by a Culture Technique and Detection by PCR

Popis výsledku anglicky

A pilot survey of sources of contamination with arcobacters, which represent a potential risk for humans, is described. A wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.), both from a retail level and domestic farming. Sanitary practices in abattoirs and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The applied method was based on a selective enrichment to isolate suspect strains,in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical for the genus Arcobacter, and a product of 686 bp, specific for A. butzleri. On the whole, a total number of 198 samples was tested, of that 33 (16.7%) were found to

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F02%2F0023" target="_blank" >GA203/02/0023: Vývoj nových postupů pro vysoce spolehlivou analyt. kontrolu technol.,materiálů,potravin a život. prostř. a pro lékařskou diag. s využitím moderní instrumentace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Svazek periodika

48

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

227-232

Kód UT WoS článku

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EID výsledku v databázi Scopus

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