Porovnání jednotlivých metod izolace DNA z aflatoxinogenních plísní.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F06%3A00004295" target="_blank" >RIV/00216275:25310/06:00004295 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of aflatoxinogenic fungal DNA isolation for PCR.

Popis výsledku v původním jazyce

Two classical techniques of DNA extraction were optimized and compared with use of commercial PCR kits. For DNA isolation was also used commercially delivered compound DNAzol(R)ES. Methods were optimized by using pure cultures A. parasiticus var. globosus CCM F - 550 and A. flavus CCM F - 108. These methods were classified in view of DNA purity, concentration, speed of isolation, intensity of instrument techniques and manipulation and last but not least were taken single isolation cost into consideration. The most effective method for DNA isolation was chosen for positive samples for the presence of the aflatoxinogenic fungi. 83 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of theaflatoxinogenic fungi on the AFPA medium. After DNA isolation was made amplification by PCR and detection by electrophoresis. Acquired result practically corresponded with result gained on the AFPA medium.

Název v anglickém jazyce

Comparison of aflatoxinogenic fungal DNA isolation for PCR.

Popis výsledku anglicky

Two classical techniques of DNA extraction were optimized and compared with use of commercial PCR kits. For DNA isolation was also used commercially delivered compound DNAzol(R)ES. Methods were optimized by using pure cultures A. parasiticus var. globosus CCM F - 550 and A. flavus CCM F - 108. These methods were classified in view of DNA purity, concentration, speed of isolation, intensity of instrument techniques and manipulation and last but not least were taken single isolation cost into consideration. The most effective method for DNA isolation was chosen for positive samples for the presence of the aflatoxinogenic fungi. 83 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of theaflatoxinogenic fungi on the AFPA medium. After DNA isolation was made amplification by PCR and detection by electrophoresis. Acquired result practically corresponded with result gained on the AFPA medium.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Moderní instrumentace v chemické, potravinářské a klinické analýze</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

20th International ICFMH Symposium Food Micro 2006

ISBN

961-90346-3-5

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

453

Název nakladatele

International Committee on Food Microbiology and Hygiene

Místo vydání

Bologna, Itálie

Místo konání akce

Bologna

Datum konání akce

29. 8. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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