Extrakce DNA z aflatoxinogenních druhů aspergilů vyskytujících se v potravinách pro PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F07%3A00005935" target="_blank" >RIV/00216275:25310/07:00005935 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method

Popis výsledku v původním jazyce

The extraction of DNA according to Cenis was used with different modifications of disrupting the cell wall. Fungi mycelium was disrupted by ultrasonic bath, Eppendorf homogenizer and liquid nitrogen. Method was optimized using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. After extraction, acquired DNA was amplified by Polymerase Chain Reaction (PCR). PCR was used to amplify ver-1 gene and apa-2 gene as target fragments. The specific PCR product codes the biosyntheticway of aflatoxin B1. PCR product was detected by electrophoresis. Records were interpreted. Acquired results demonstrate, that success of method depends especially on disrupting cell wall of fungi. 91 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. Two samples contained two species fungi of genus Aspergillus and one sample contained three species fungi of genus Aspergil

Název v anglickém jazyce

Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method

Popis výsledku anglicky

The extraction of DNA according to Cenis was used with different modifications of disrupting the cell wall. Fungi mycelium was disrupted by ultrasonic bath, Eppendorf homogenizer and liquid nitrogen. Method was optimized using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. After extraction, acquired DNA was amplified by Polymerase Chain Reaction (PCR). PCR was used to amplify ver-1 gene and apa-2 gene as target fragments. The specific PCR product codes the biosyntheticway of aflatoxin B1. PCR product was detected by electrophoresis. Records were interpreted. Acquired results demonstrate, that success of method depends especially on disrupting cell wall of fungi. 91 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. Two samples contained two species fungi of genus Aspergillus and one sample contained three species fungi of genus Aspergil

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Papers of the University of Pardubice, Series A

ISSN

1211-5541

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

13

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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