Short-term stability of biomarkers of oxidative stress and antioxidant status in human serum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896517" target="_blank" >RIV/00216275:25310/13:39896517 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1155/4174" target="_blank" >http://dx.doi.org/10.1155/4174</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/4174" target="_blank" >10.1155/4174</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Short-term stability of biomarkers of oxidative stress and antioxidant status in human serum

Popis výsledku v původním jazyce

The oxidation and antioxidant status of serum are often determined in serum samples which have been frozen for some time. The oxidative stress process is prone to fast alterations in the sample because of the possible instability of the reactants. Here one oxidation assay (ROM) and three antioxidant assays (FRAP, TAS, and BAP) have been tested on their performance and stability at short-time storage. The most commonly used temperatures for storage and handling of serum samples (+4 and +20?C) were selected. In three short-term studies in which the storage time varied between 3 and 48 hrs the performance of these assays were tested on human serum samples. The general conclusion is that most assays performed well and gave stable results during 2 days of storage of the samples at both temperatures. Only the FRAP and TAS assays showed a small deviation at some storage conditions. In conclusion, handling of serum samples at +4 and +20?C during short-time periods did not affect the quality an

Název v anglickém jazyce

Short-term stability of biomarkers of oxidative stress and antioxidant status in human serum

Popis výsledku anglicky

The oxidation and antioxidant status of serum are often determined in serum samples which have been frozen for some time. The oxidative stress process is prone to fast alterations in the sample because of the possible instability of the reactants. Here one oxidation assay (ROM) and three antioxidant assays (FRAP, TAS, and BAP) have been tested on their performance and stability at short-time storage. The most commonly used temperatures for storage and handling of serum samples (+4 and +20?C) were selected. In three short-term studies in which the storage time varied between 3 and 48 hrs the performance of these assays were tested on human serum samples. The general conclusion is that most assays performed well and gave stable results during 2 days of storage of the samples at both temperatures. Only the FRAP and TAS assays showed a small deviation at some storage conditions. In conclusion, handling of serum samples at +4 and +20?C during short-time periods did not affect the quality an

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ISRN Biomarkers

ISSN

2314-6265

e-ISSN

—

Svazek periodika

2013

Číslo periodika v rámci svazku

2013

Stát vydavatele periodika

EG - Egyptská arabská republika

Počet stran výsledku

5

Strana od-do

1-5

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—