Long-term stability of cancer biomarkers in human serum: biomarkers of oxidative stress and redox status, homocysteine, CRP and the enzymes ALT and GGT

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39899905" target="_blank" >RIV/00216275:25310/15:39899905 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2217/bmm.15.14" target="_blank" >http://dx.doi.org/10.2217/bmm.15.14</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2217/bmm.15.14" target="_blank" >10.2217/bmm.15.14</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Long-term stability of cancer biomarkers in human serum: biomarkers of oxidative stress and redox status, homocysteine, CRP and the enzymes ALT and GGT

Popis výsledku v původním jazyce

Aim: Five frequently used biomarkers in cancer research and epidemiological studies were tested for their assay stability upon storage of serum for 12 months at -20 and -70/-80 degrees C. Materials & methods: The biomarker assays include reactive oxygenmetabolites (ROM), the total thiol levels (TTL), homocysteine (HCy), C-reactive protein (HS-CRP) and two liver enzymes, alanine aminotransferase (ALT) and -glutamyltransferase (GGT). Results: The assays for ROM, HCy, HS-CRP and GGT were stable in human serum samples at the two temperatures tested. The two other assays TTL and ALT, however, showed statistically significant differences in their stability between -20 and -80 degrees C. Conclusion: Therefore, storage at -80 degrees C is advised to maintaina reliable assay outcome when serum samples have to be stored for longer periods.

Název v anglickém jazyce

Long-term stability of cancer biomarkers in human serum: biomarkers of oxidative stress and redox status, homocysteine, CRP and the enzymes ALT and GGT

Popis výsledku anglicky

Aim: Five frequently used biomarkers in cancer research and epidemiological studies were tested for their assay stability upon storage of serum for 12 months at -20 and -70/-80 degrees C. Materials & methods: The biomarker assays include reactive oxygenmetabolites (ROM), the total thiol levels (TTL), homocysteine (HCy), C-reactive protein (HS-CRP) and two liver enzymes, alanine aminotransferase (ALT) and -glutamyltransferase (GGT). Results: The assays for ROM, HCy, HS-CRP and GGT were stable in human serum samples at the two temperatures tested. The two other assays TTL and ALT, however, showed statistically significant differences in their stability between -20 and -80 degrees C. Conclusion: Therefore, storage at -80 degrees C is advised to maintaina reliable assay outcome when serum samples have to be stored for longer periods.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomarkers in Medicine

ISSN

1752-0363

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

425-432

Kód UT WoS článku

000354647700004

EID výsledku v databázi Scopus

—