Long-term stability of oxidative stress biomarkers in human serum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39910902" target="_blank" >RIV/00216275:25310/17:39910902 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/10715762.2017.1398403" target="_blank" >http://dx.doi.org/10.1080/10715762.2017.1398403</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/10715762.2017.1398403" target="_blank" >10.1080/10715762.2017.1398403</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Long-term stability of oxidative stress biomarkers in human serum

Popis výsledku v původním jazyce

The storage time and storage temperature might affect stability of oxidative stress biomarkers, therefore, they have to be analyzed after long-term storage of serum samples. The stability of three biomarkers reflecting oxidative stress: reactive oxygen metabolites (ROM) for hydro peroxides, total thiol levels (TTL) for the redox status and biological antioxidant potency (BAP) for the antioxidant status, was investigated at several time points during 60 months of storage at -20 and -80°C. Biomarkers ROM and BAP showed a very good stability during storage for 60 months at both temperatures. In addition, the correlation of the data after 60 months of storage compared with the starting data was very good with correlation coefficients &gt;0.9. The TTL assay showed good results in serum samples stored at -80°C, but not in samples stored at -20°C. Serum samples for analysis of the set of oxidative stress biomarkers ROM, BAP and TTL can be stored up to 60 months at -80°C. ROM and BAP can also be stored at -20°C during this period. The present results are very important for the biomarker-related epidemiological studies that make use of biobanks with samples stored for many years and for new project planning, including sample storage conditions.

Název v anglickém jazyce

Long-term stability of oxidative stress biomarkers in human serum

Popis výsledku anglicky

The storage time and storage temperature might affect stability of oxidative stress biomarkers, therefore, they have to be analyzed after long-term storage of serum samples. The stability of three biomarkers reflecting oxidative stress: reactive oxygen metabolites (ROM) for hydro peroxides, total thiol levels (TTL) for the redox status and biological antioxidant potency (BAP) for the antioxidant status, was investigated at several time points during 60 months of storage at -20 and -80°C. Biomarkers ROM and BAP showed a very good stability during storage for 60 months at both temperatures. In addition, the correlation of the data after 60 months of storage compared with the starting data was very good with correlation coefficients &gt;0.9. The TTL assay showed good results in serum samples stored at -80°C, but not in samples stored at -20°C. Serum samples for analysis of the set of oxidative stress biomarkers ROM, BAP and TTL can be stored up to 60 months at -80°C. ROM and BAP can also be stored at -20°C during this period. The present results are very important for the biomarker-related epidemiological studies that make use of biobanks with samples stored for many years and for new project planning, including sample storage conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30107 - Medicinal chemistry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Free Radical Research

ISSN

1071-5762

e-ISSN

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Svazek periodika

51

Číslo periodika v rámci svazku

11-12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

970-977

Kód UT WoS článku

000416652800008

EID výsledku v databázi Scopus

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