PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39897856" target="_blank" >RIV/00216275:25310/14:39897856 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/14:00436781

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.msec.2014.04.011" target="_blank" >http://dx.doi.org/10.1016/j.msec.2014.04.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2014.04.011" target="_blank" >10.1016/j.msec.2014.04.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

Popis výsledku v původním jazyce

In this study, magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (PGMA-COOH) were coated using highly hydrophilic polymer poly( ethylene glycol) (PEG). PEG was used to reduce nonspecific interactions with proteins and cells while decreasing adhesion of particles to the walls of a microfluidic devices from poly(dimethylsiloxane) (PDMS) and cyclic olefin copolymer (COC). Zeta potential measurement, infrared spectroscopy, scanning electron microscopy, anti-PEG ELISA assay, andbioaffinity interactions between biotin and streptavidin-HRP successfully proved the presence of PEG on the surface of microspheres. Both neat and PEGylated microspheres were then incubated with the inert protein bovine serum albumin or cells to evaluatethe rate of nonspecific adsorption (NSA). PEG with Mr of 30,000 Da was responsible for 45% reduction in NSA of proteins and 74% for cells compared to neat particles. The microspheres' behavior in PDMS and COC microchannels was then evalu

Název v anglickém jazyce

PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

Popis výsledku anglicky

In this study, magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (PGMA-COOH) were coated using highly hydrophilic polymer poly( ethylene glycol) (PEG). PEG was used to reduce nonspecific interactions with proteins and cells while decreasing adhesion of particles to the walls of a microfluidic devices from poly(dimethylsiloxane) (PDMS) and cyclic olefin copolymer (COC). Zeta potential measurement, infrared spectroscopy, scanning electron microscopy, anti-PEG ELISA assay, andbioaffinity interactions between biotin and streptavidin-HRP successfully proved the presence of PEG on the surface of microspheres. Both neat and PEGylated microspheres were then incubated with the inert protein bovine serum albumin or cells to evaluatethe rate of nonspecific adsorption (NSA). PEG with Mr of 30,000 Da was responsible for 45% reduction in NSA of proteins and 74% for cells compared to neat particles. The microspheres' behavior in PDMS and COC microchannels was then evalu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Science & Engineering C: Materials for Biological Applications

ISSN

0928-4931

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

308-315

Kód UT WoS článku

000338388400041

EID výsledku v databázi Scopus

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