Determination of Total Phenolic Content in Selected Wines Using Amperometric Tyrosinase Biosensor Based on Carbon Nanotubes

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of Total Phenolic Content in Selected Wines Using Amperometric Tyrosinase Biosensor Based on Carbon Nanotubes

Popis výsledku v původním jazyce

Polyphenols exhibit significant antioxidant properties of wines. Their antioxidant capacity is commonly expressed as the equivalent weight of Trolox which is synthetic water-soluble analog of alfa-tocopherol (vitamin E), and is called "Trolox equivalentantioxidant capacity" (TEAC). This contribution offers a direct determination of TEAC in real samples without the use of free radicals as it is in conventional spectrometric methods. An amperometric tyrosinase biosensor based on carbon paste electrode modified with carbon nanotubes (CPE/CNTs) is presented. The biosensor, CPE/CNTs/Tyrosinase/Nafion, was prepared by immobilization of tyrosinase enzyme together with Nafion on the surface of CPE/CNTs. All key experimental and instrumentals parameters have been investigated and optimal conditions were founded as follows: (i) amount of tyrosinase in Nafion membrane, 3.0 ?g; (ii) operating potential for amperometric detection, -0.25 V; (iii) speed of stirring, aprox. 400 rpm; (iv) supporting e

Název v anglickém jazyce

Determination of Total Phenolic Content in Selected Wines Using Amperometric Tyrosinase Biosensor Based on Carbon Nanotubes

Popis výsledku anglicky

Polyphenols exhibit significant antioxidant properties of wines. Their antioxidant capacity is commonly expressed as the equivalent weight of Trolox which is synthetic water-soluble analog of alfa-tocopherol (vitamin E), and is called "Trolox equivalentantioxidant capacity" (TEAC). This contribution offers a direct determination of TEAC in real samples without the use of free radicals as it is in conventional spectrometric methods. An amperometric tyrosinase biosensor based on carbon paste electrode modified with carbon nanotubes (CPE/CNTs) is presented. The biosensor, CPE/CNTs/Tyrosinase/Nafion, was prepared by immobilization of tyrosinase enzyme together with Nafion on the surface of CPE/CNTs. All key experimental and instrumentals parameters have been investigated and optimal conditions were founded as follows: (i) amount of tyrosinase in Nafion membrane, 3.0 ?g; (ii) operating potential for amperometric detection, -0.25 V; (iii) speed of stirring, aprox. 400 rpm; (iv) supporting e

Druh

D - Stať ve sborníku

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník přednášek mezinárodní odborné konference XXXIV. Moderní elektrochemické metody

ISBN

978-80-905221-2-1

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

176-180

Název nakladatele

BEST Servis

Místo vydání

Ústí nad Labem

Místo konání akce

Jetřichovice

Datum konání akce

19. 5. 2014

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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