Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917763" target="_blank" >RIV/62156489:43210/20:43917763 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/20:00525437 RIV/00216275:25310/20:39916073 RIV/00216305:26620/20:PU136746

Výsledek na webu

<a href="https://doi.org/10.3390/app10072497" target="_blank" >https://doi.org/10.3390/app10072497</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/app10072497" target="_blank" >10.3390/app10072497</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes

Popis výsledku v původním jazyce

In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion(R) layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well.

Název v anglickém jazyce

Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes

Popis výsledku anglicky

In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion(R) layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA19-03160S" target="_blank" >GA19-03160S: Elektrochemická studie nových umělých enzymů a jejich role v analýze neurotransmiterů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Sciences

ISSN

2076-3417

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

2497

Kód UT WoS článku

000533356200296

EID výsledku v databázi Scopus

2-s2.0-85083460750