Improvement of Enzyme Carbon Paste-Based Biosensor Using Carbon Nanotubes for Determination of Water Soluble Analogue of Vitamin E

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900622" target="_blank" >RIV/00216275:25310/15:39900622 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.degruyter.com//view/j/chempap.2015.69.issue-1/s11696-014-0608-9/s11696-014-0608-9.xml" target="_blank" >http://www.degruyter.com//view/j/chempap.2015.69.issue-1/s11696-014-0608-9/s11696-014-0608-9.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11696-014-0608-9" target="_blank" >10.2478/s11696-014-0608-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Improvement of Enzyme Carbon Paste-Based Biosensor Using Carbon Nanotubes for Determination of Water Soluble Analogue of Vitamin E

Popis výsledku v původním jazyce

The catalytic oxidation of a synthetic water-soluble analogue of vitamin E (?-tocopherol, Trolox) by tyrosinase enzyme in the presence of molecular oxygen was studied using electrochemical techniques. This specific enzymatic reaction was exploited for the preparation of a biosensor based on the amperometric reduction of the electroactive product (?-tocoquinone) formed. An electroactive surface of the transducers used was covered with a thin conductive layer of Nafion containing tyrosinase. Significant progress in sensitivity towards polyphenolic compounds such as Trolox was achieved at CPE with carbon nanotubes immobilised on its surface (CPE/CNTs) as electric transducers. The biosensor so developed can be used for the direct determination of total phenolic content (TPC). This important nutrition value can be expressed as the mass equivalent of Trolox, i.e. Trolox equivalent antioxidant capacity (TEAC), which could be used as an alternative to the evaluations currently used based on sp

Název v anglickém jazyce

Improvement of Enzyme Carbon Paste-Based Biosensor Using Carbon Nanotubes for Determination of Water Soluble Analogue of Vitamin E

Popis výsledku anglicky

The catalytic oxidation of a synthetic water-soluble analogue of vitamin E (?-tocopherol, Trolox) by tyrosinase enzyme in the presence of molecular oxygen was studied using electrochemical techniques. This specific enzymatic reaction was exploited for the preparation of a biosensor based on the amperometric reduction of the electroactive product (?-tocoquinone) formed. An electroactive surface of the transducers used was covered with a thin conductive layer of Nafion containing tyrosinase. Significant progress in sensitivity towards polyphenolic compounds such as Trolox was achieved at CPE with carbon nanotubes immobilised on its surface (CPE/CNTs) as electric transducers. The biosensor so developed can be used for the direct determination of total phenolic content (TPC). This important nutrition value can be expressed as the mass equivalent of Trolox, i.e. Trolox equivalent antioxidant capacity (TEAC), which could be used as an alternative to the evaluations currently used based on sp

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.30.0021" target="_blank" >EE2.3.30.0021: Posílení excelentních týmů výzkumu a vývoje na Univerzitě Pardubice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Papers

ISSN

0366-6352

e-ISSN

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Svazek periodika

69

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

150-157

Kód UT WoS článku

000350130100013

EID výsledku v databázi Scopus

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