Simultaneous determination of phenylalanine and tyrosine levels in human blood obtained by the dried spot technique for monitoring of patients with phenylketonuria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F25%3A39922832" target="_blank" >RIV/00216275:25310/25:39922832 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.jpba.2025.116831" target="_blank" >https://doi.org/10.1016/j.jpba.2025.116831</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jpba.2025.116831" target="_blank" >10.1016/j.jpba.2025.116831</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous determination of phenylalanine and tyrosine levels in human blood obtained by the dried spot technique for monitoring of patients with phenylketonuria

Popis výsledku v původním jazyce

Monitoring patients with phenylketonuria (PKU) requires accurately measuring phenylalanine and tyrosine levels in a small volume of blood samples obtained by the dried blood spot (DBS) technique. The aim was to study selected parameters influencing the quantitative results. Phenylalanine and tyrosine were extracted from DBS samples with methanol, and 5 internal standard introduction techniques were tested. Phenylalanine and tyrosine levels were measured in 6-mm discs punched from DBS, pre-punched 9-mm discs containing the entire DBS sample, and liquid blood by HPLC-MS-MS. Levels in 6-mm discs punched from DBS measured by HPLC-MS/MS were compared with those measured by the HPLC-FLD. The analytical parameters of the method are satisfactory, linearity in the range of 25–1200 μmol/L (LOD, LOQ and LLOQ values 0.2 μmol/L, 0.5 μmol/L and 3.8 μmol/L for phenylalanine, 0.5 μmol/L, 1.5 μmol/L and 5.1 μmol/L for tyrosine), within-run precision 1.8 %–3.7 % for phenylalanine, 1.9 %–2.7 % for tyrosine, between-run precision 4.7 %–5.9 % for phenylalanine, 4.1 %–5.4 % for tyrosine, recovery 93.8 %–100.4 % for phenylalanine and 93.7 %–99.1 % for tyrosine. Good agreement was found between phenylalanine and tyrosine concentrations in 6-mm discs punched from DBS (R = 0.896, p &lt; 0.001, and R = 0.907, p &lt; 0.001, respectively), pre-punched 9-mm discs containing the entire DBS sample (R = 0.960, p &lt; 0.001, and R = 0.950, p &lt; 0.001, respectively) and liquid blood, as well as between phenylalanine and tyrosine concentrations obtained by HPLC-MS/MS and HPLC-FLD (R = 0.968, p &lt; 0.001, and R = 0.984, p &lt; 0.001, respectively). The presented method is suitable for monitoring patients with PKU.

Název v anglickém jazyce

Simultaneous determination of phenylalanine and tyrosine levels in human blood obtained by the dried spot technique for monitoring of patients with phenylketonuria

Popis výsledku anglicky

Monitoring patients with phenylketonuria (PKU) requires accurately measuring phenylalanine and tyrosine levels in a small volume of blood samples obtained by the dried blood spot (DBS) technique. The aim was to study selected parameters influencing the quantitative results. Phenylalanine and tyrosine were extracted from DBS samples with methanol, and 5 internal standard introduction techniques were tested. Phenylalanine and tyrosine levels were measured in 6-mm discs punched from DBS, pre-punched 9-mm discs containing the entire DBS sample, and liquid blood by HPLC-MS-MS. Levels in 6-mm discs punched from DBS measured by HPLC-MS/MS were compared with those measured by the HPLC-FLD. The analytical parameters of the method are satisfactory, linearity in the range of 25–1200 μmol/L (LOD, LOQ and LLOQ values 0.2 μmol/L, 0.5 μmol/L and 3.8 μmol/L for phenylalanine, 0.5 μmol/L, 1.5 μmol/L and 5.1 μmol/L for tyrosine), within-run precision 1.8 %–3.7 % for phenylalanine, 1.9 %–2.7 % for tyrosine, between-run precision 4.7 %–5.9 % for phenylalanine, 4.1 %–5.4 % for tyrosine, recovery 93.8 %–100.4 % for phenylalanine and 93.7 %–99.1 % for tyrosine. Good agreement was found between phenylalanine and tyrosine concentrations in 6-mm discs punched from DBS (R = 0.896, p &lt; 0.001, and R = 0.907, p &lt; 0.001, respectively), pre-punched 9-mm discs containing the entire DBS sample (R = 0.960, p &lt; 0.001, and R = 0.950, p &lt; 0.001, respectively) and liquid blood, as well as between phenylalanine and tyrosine concentrations obtained by HPLC-MS/MS and HPLC-FLD (R = 0.968, p &lt; 0.001, and R = 0.984, p &lt; 0.001, respectively). The presented method is suitable for monitoring patients with PKU.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Pharmaceutical and Biomedical Analysis

ISSN

0731-7085

e-ISSN

1873-264X

Svazek periodika

260

Číslo periodika v rámci svazku

August 2025

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

116831

Kód UT WoS článku

001454117200001

EID výsledku v databázi Scopus

2-s2.0-105000487145