The Application of PCR Methods for Clostridium Identification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F09%3APU84180" target="_blank" >RIV/00216305:26310/09:PU84180 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Application of PCR Methods for Clostridium Identification

Popis výsledku v původním jazyce

The presence of butyric acid bacteria and spores in milk causes problems in cheese technology. Some species of genus Clostridium provoke the late-blowing effect which is characterized by high concentration of butyric acid and formation of gas holes in cheese. This work is focused on application of PCR methods for genus and species identification of selected strains of Clostridium. Using genus-specific PCR, the set of 44 DNA samples was identified as DNA of Clostridium genus. Based on species-specific PCRs, it was found out that 7 samples belong to the species Clostridium butyricum and 12 samples belong to the species Clostridium tyrobutyricum. Fifteen samples were positively detected using both species-specific PCRs and therefore they were identified as DNA mixtures from both cultures. Another 10 samples were not classified into tested species. Strains with the hydrogenase (hydA) gene encoding H2-evolving hydrogenase were also searched using PCR. Specific PCR products were detected in

Název v anglickém jazyce

The Application of PCR Methods for Clostridium Identification

Popis výsledku anglicky

The presence of butyric acid bacteria and spores in milk causes problems in cheese technology. Some species of genus Clostridium provoke the late-blowing effect which is characterized by high concentration of butyric acid and formation of gas holes in cheese. This work is focused on application of PCR methods for genus and species identification of selected strains of Clostridium. Using genus-specific PCR, the set of 44 DNA samples was identified as DNA of Clostridium genus. Based on species-specific PCRs, it was found out that 7 samples belong to the species Clostridium butyricum and 12 samples belong to the species Clostridium tyrobutyricum. Fifteen samples were positively detected using both species-specific PCRs and therefore they were identified as DNA mixtures from both cultures. Another 10 samples were not classified into tested species. Strains with the hydrogenase (hydA) gene encoding H2-evolving hydrogenase were also searched using PCR. Specific PCR products were detected in

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/2B08070" target="_blank" >2B08070: Vývoj nového moderního biotechnologického postupu umožňujícího účinnější způsob využívání odpadních vod z mlékárenského průmyslu jako materiálového zdroje pro výrobu vodíku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Nova Biotechnologica, Proceedings of the International Conference Applied Natural Sciences 2009

ISBN

978-80-8105-129-6

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

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Název nakladatele

University of SS. Cyril and Methodius in Trnava

Místo vydání

Nám. J. Herdu 2, Trnava

Místo konání akce

Trnava

Datum konání akce

7. 10. 2009

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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