PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F11%3APU94888" target="_blank" >RIV/00216305:26310/11:PU94888 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine

Popis výsledku v původním jazyce

The aim of this study was to identify yeasts isolated from red wine of Rulandske modre variety (Vitis vinifera) from integrated and ecological agriculture mode (production) from South Moravia. The yeast strains were isolated in regular intervals during spontaneous fermented must (without any SO2 addition). Together, we identified 124 strains (60 from ecological; 64 from integrated fermented must). As a rapid identification method, we used PCR-RFLP (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism) and by application of specific primers (ITS1-ITS4) we amplified 5.8S-ITS segment of rDNA. PCR-RFLP of ITS regions using HaeIII, HinfI, TaqI, AluI and MseI (TruI) was used for identification and grouping of genetically similar strains. Restriction fragments were detected by agarose gel electrophoresis. All obtained electrophoreograms were processed by software BioNumerics 6.5 and UPGMA cluster analysis.

Název v anglickém jazyce

PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine

Popis výsledku anglicky

The aim of this study was to identify yeasts isolated from red wine of Rulandske modre variety (Vitis vinifera) from integrated and ecological agriculture mode (production) from South Moravia. The yeast strains were isolated in regular intervals during spontaneous fermented must (without any SO2 addition). Together, we identified 124 strains (60 from ecological; 64 from integrated fermented must). As a rapid identification method, we used PCR-RFLP (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism) and by application of specific primers (ITS1-ITS4) we amplified 5.8S-ITS segment of rDNA. PCR-RFLP of ITS regions using HaeIII, HinfI, TaqI, AluI and MseI (TruI) was used for identification and grouping of genetically similar strains. Restriction fragments were detected by agarose gel electrophoresis. All obtained electrophoreograms were processed by software BioNumerics 6.5 and UPGMA cluster analysis.

Druh

O - Ostatní výsledky

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů