The Use of Bioactive Compounds and Their Encapsulation into Liposomes to Increase the Effectiveness and Control Release

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F18%3APU130885" target="_blank" >RIV/00216305:26310/18:PU130885 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Use of Bioactive Compounds and Their Encapsulation into Liposomes to Increase the Effectiveness and Control Release

Popis výsledku v původním jazyce

The aim of this work is to encapsulate microbial compounds, some plant extracts and also model hydrophilic and hydrophobic substances. Chosen components were encapsulated into liposomes. Particle size and distributions of liposomes were analyzed by dynamic light scattering and their stability by using zeta potential. Spectrophotometric methods were used to measure encapsulation efficiency and to characterize the chosen plant extracts by its amount of total polyphenols, flavonoids and antioxidant activity. Chosen samples were analyzed for their antimicrobial activity and cytotoxicity. Serratia marcescens, Escherichia coli as gram-negative bacterias, Micrococcus luteus as gram-positive bacteria and fungal strain (Candida glabrata) were used to test antimicrobial activity. Human keratinocytes and mouse melanoma cells were used to cytotoxicity determination.

Název v anglickém jazyce

The Use of Bioactive Compounds and Their Encapsulation into Liposomes to Increase the Effectiveness and Control Release

Popis výsledku anglicky

The aim of this work is to encapsulate microbial compounds, some plant extracts and also model hydrophilic and hydrophobic substances. Chosen components were encapsulated into liposomes. Particle size and distributions of liposomes were analyzed by dynamic light scattering and their stability by using zeta potential. Spectrophotometric methods were used to measure encapsulation efficiency and to characterize the chosen plant extracts by its amount of total polyphenols, flavonoids and antioxidant activity. Chosen samples were analyzed for their antimicrobial activity and cytotoxicity. Serratia marcescens, Escherichia coli as gram-negative bacterias, Micrococcus luteus as gram-positive bacteria and fungal strain (Candida glabrata) were used to test antimicrobial activity. Human keratinocytes and mouse melanoma cells were used to cytotoxicity determination.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10609 - Biochemical research methods

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů