Attenuation of p53-alpha isoform transactivation by inverted repeat sequences in p53 target sites
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU136159" target="_blank" >RIV/00216305:26310/19:PU136159 - isvavai.cz</a>
Výsledek na webu
<a href="https://2019.febscongress.org/abstract_preview.aspx?idAbstractEnc=4424170094096098094092424170" target="_blank" >https://2019.febscongress.org/abstract_preview.aspx?idAbstractEnc=4424170094096098094092424170</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Attenuation of p53-alpha isoform transactivation by inverted repeat sequences in p53 target sites
Popis výsledku v původním jazyce
p53 is one of the most studied tumour suppressor proteins, playing important roles in regulating basic biological processes including cell cycle, apoptosis, senescenceand metabolism. The human Tp53 gene contains alternative promoters and thanks to alternative splicing can produce several isoforms. p53 protein function is realizedby binding to specific DNA response elements resulting in the transactivation of target genes. Here we present results of p53alpha isoform obtained using a yeastisogenic system for in vivo transactivation studies in chromosomal context to specifically evaluate the influence of secondary DNA structures on transactivation. We useda panel of S. cerevisiaehaploid strains that are isogenic except for different p53 DNA binding sites positioned upstream of a luciferase reporter gene and chosen basedon different propensities to form DNA structures. The targeting of the chosen p53 binding site was achieved by the Delitto Perfettooligonucleotide targeting techniqueby the replacement of
Název v anglickém jazyce
Attenuation of p53-alpha isoform transactivation by inverted repeat sequences in p53 target sites
Popis výsledku anglicky
p53 is one of the most studied tumour suppressor proteins, playing important roles in regulating basic biological processes including cell cycle, apoptosis, senescenceand metabolism. The human Tp53 gene contains alternative promoters and thanks to alternative splicing can produce several isoforms. p53 protein function is realizedby binding to specific DNA response elements resulting in the transactivation of target genes. Here we present results of p53alpha isoform obtained using a yeastisogenic system for in vivo transactivation studies in chromosomal context to specifically evaluate the influence of secondary DNA structures on transactivation. We useda panel of S. cerevisiaehaploid strains that are isogenic except for different p53 DNA binding sites positioned upstream of a luciferase reporter gene and chosen basedon different propensities to form DNA structures. The targeting of the chosen p53 binding site was achieved by the Delitto Perfettooligonucleotide targeting techniqueby the replacement of
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/LO1211" target="_blank" >LO1211: Centrum materiálového výzkumu na FCH VUT v Brně - udržitelnost a rozvoj</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů