The influence of non-canonical structures on the p53 isoform binding to DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F20%3APU137934" target="_blank" >RIV/00216305:26310/20:PU137934 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The influence of non-canonical structures on the p53 isoform binding to DNA

Popis výsledku v původním jazyce

Protein p53 is one of the most studied tumor suppressor protein plays important roles in regulating basic biological processes including cell cycle, apoptosis, senescence and metabolism. The human p53 gene contains alternative promoters and thank to alternative splicing could be expressed in several isoforms. P53 protein function is realized by binding to specific DNA response elements (RE) and transactivation of target genes. Here we presented results of p53alpha, p53beta and p53gamma isoforms DNA interaction studied by yeast isogenic system for in vivo transactivation studies in chromosomal structures to protein-DNA binding. WE used a panel of Saccharomyces cerevisiae haploid isogenic strains, except for the different p53 target site with propensity to form different DNA structures located upstream of the luciferase reporter gene. The targeting of p53 target sequence of interest by the replacement of the ICORE cassette, using transfected single strand oligonucleotides, was performed following the De

Název v anglickém jazyce

The influence of non-canonical structures on the p53 isoform binding to DNA

Popis výsledku anglicky

Protein p53 is one of the most studied tumor suppressor protein plays important roles in regulating basic biological processes including cell cycle, apoptosis, senescence and metabolism. The human p53 gene contains alternative promoters and thank to alternative splicing could be expressed in several isoforms. P53 protein function is realized by binding to specific DNA response elements (RE) and transactivation of target genes. Here we presented results of p53alpha, p53beta and p53gamma isoforms DNA interaction studied by yeast isogenic system for in vivo transactivation studies in chromosomal structures to protein-DNA binding. WE used a panel of Saccharomyces cerevisiae haploid isogenic strains, except for the different p53 target site with propensity to form different DNA structures located upstream of the luciferase reporter gene. The targeting of p53 target sequence of interest by the replacement of the ICORE cassette, using transfected single strand oligonucleotides, was performed following the De

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů