The influence of non-canonical structures on the p53 isoforms binding to DNA
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F20%3APU137933" target="_blank" >RIV/00216305:26310/20:PU137933 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The influence of non-canonical structures on the p53 isoforms binding to DNA
Popis výsledku v původním jazyce
Protein p53 is one of the most studied tumor suppressor protein plays important roles in regulating basic biological processes including cell cycle, apoptosis, senescence and metabolism. The human p53 gene contains alternative promoters and thank to alternative splicing could be expressed in several isoforms. P53 protein function is realized by binding to specific DNA response elements (RE) and transactivation of target genes. Here we presented results of p53alpha, p53beta and p53gamma isoforms DNA interaction studied by yeast isogenic system for in vivo transactivation studies in chromosomal structures to protein-DNA binding. WE used a panel of Saccharomyces cerevisiae haploid isogenic strains, except for the different p53 target site with propensity to form different DNA structures located upstream of the luciferase reporter gene. The targeting of p53 target sequence of interest by the replacement of the ICORE cassette, using transfected single strand oligonucleotides, was performed following the De
Název v anglickém jazyce
The influence of non-canonical structures on the p53 isoforms binding to DNA
Popis výsledku anglicky
Protein p53 is one of the most studied tumor suppressor protein plays important roles in regulating basic biological processes including cell cycle, apoptosis, senescence and metabolism. The human p53 gene contains alternative promoters and thank to alternative splicing could be expressed in several isoforms. P53 protein function is realized by binding to specific DNA response elements (RE) and transactivation of target genes. Here we presented results of p53alpha, p53beta and p53gamma isoforms DNA interaction studied by yeast isogenic system for in vivo transactivation studies in chromosomal structures to protein-DNA binding. WE used a panel of Saccharomyces cerevisiae haploid isogenic strains, except for the different p53 target site with propensity to form different DNA structures located upstream of the luciferase reporter gene. The targeting of p53 target sequence of interest by the replacement of the ICORE cassette, using transfected single strand oligonucleotides, was performed following the De
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů