Slow growth in vitro conservation of fruit crops

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000093" target="_blank" >RIV/25271121:_____/19:N0000093 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.15" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1234.15</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.15" target="_blank" >10.17660/ActaHortic.2019.1234.15</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Slow growth in vitro conservation of fruit crops

Popis výsledku v původním jazyce

Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd. holds a fruit germplasm of about 2500 accessions in field collections. Unfortunately trees in these collections are at permanent risk from environmental conditions, pests and diseases. Germplasm preservation of vegetatively propagated fruit species in field collections may be supplemented using biotechnological methods. In vitro storage under slow-growth conditions is one of these techniques. The experiment with medium term storage of in vitro cultures of strawberry, apple, pear and sweet cherry in low above zero temperature 4.0 °C was used in RBIP Holovousy Ltd. as a duplicate method to field collections. In vitro cultures were established on MS medium supplemented with 1 mg · L-1 6-benzylaminopurine (BAP) in aluminium-capped 100 ml Erlenmeyer flasks with 5 explants/25 ml of medium. The experiment was successful and viable shoot tips were obtained at the end of storage period. The best overall results were obtained with sweet cherry rootstock genotype P-HL-A, where 92.0 % explants survived without an intervening subculture after seven months. On contrast, the lowest survival was observed with sweet cherry P-HL-C, where only 2.0 % of shoots survived 7 months from the beginning of experiment. Remaining genotypes of fruit species used in the experiment had intermediate results. The storage of in vitro cultures at 4 °C appears to be promising technique as a duplicate medium-term conservation method for fruit germplasm, which can considerably reduce labor and material costs. Moreover in vitro collections are year-long accessible form of germplasm for distribution among germplasm curators and user community.

Název v anglickém jazyce

Slow growth in vitro conservation of fruit crops

Popis výsledku anglicky

Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd. holds a fruit germplasm of about 2500 accessions in field collections. Unfortunately trees in these collections are at permanent risk from environmental conditions, pests and diseases. Germplasm preservation of vegetatively propagated fruit species in field collections may be supplemented using biotechnological methods. In vitro storage under slow-growth conditions is one of these techniques. The experiment with medium term storage of in vitro cultures of strawberry, apple, pear and sweet cherry in low above zero temperature 4.0 °C was used in RBIP Holovousy Ltd. as a duplicate method to field collections. In vitro cultures were established on MS medium supplemented with 1 mg · L-1 6-benzylaminopurine (BAP) in aluminium-capped 100 ml Erlenmeyer flasks with 5 explants/25 ml of medium. The experiment was successful and viable shoot tips were obtained at the end of storage period. The best overall results were obtained with sweet cherry rootstock genotype P-HL-A, where 92.0 % explants survived without an intervening subculture after seven months. On contrast, the lowest survival was observed with sweet cherry P-HL-C, where only 2.0 % of shoots survived 7 months from the beginning of experiment. Remaining genotypes of fruit species used in the experiment had intermediate results. The storage of in vitro cultures at 4 °C appears to be promising technique as a duplicate medium-term conservation method for fruit germplasm, which can considerably reduce labor and material costs. Moreover in vitro collections are year-long accessible form of germplasm for distribution among germplasm curators and user community.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/LO1608" target="_blank" >LO1608: Výzkumné ovocnářské centrum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

9789462612310

ISSN

—

e-ISSN

—

Počet stran výsledku

6

Strana od-do

119-124

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Bangkok; Thailand

Datum konání akce

26. 3. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—