A new allelic discrimination real-time PCR assay for PavMYB10.1 genotyping to predict sweet cherry fruit colour: a comparison with the Pav-Rf-SSR assay

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F21%3AN0000158" target="_blank" >RIV/25271121:_____/21:N0000158 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.tandfonline.com/doi/full/10.1080/14620316.2020.1837021" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/14620316.2020.1837021</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/14620316.2020.1837021 " target="_blank" >10.1080/14620316.2020.1837021 </a>

Jazyk výsledku

angličtina

Název v původním jazyce

A new allelic discrimination real-time PCR assay for PavMYB10.1 genotyping to predict sweet cherry fruit colour: a comparison with the Pav-Rf-SSR assay

Popis výsledku v původním jazyce

Fruit skin colour in cherries is one of the most important market characteristics with customer preferences varying across cultures and countries. Based on skin colour, cherries can be divided into three market classes: yellow, blush, and mahogany. The PavMYB10.1 gene has been recently shown to be a key player in fruit colouration, and three alleles (PavMYB10.1a [WT (5A)]; PavMYB10.1b [Mut(4A)]; and PavMYB10.1c [InDel]) have been associated with cherry skin colour. In the present study, an allelic discrimination real-time PCR assay was developed for marker-assisted selection (MAS). The Pav-Rf-SSR assay that had previously been described for colour prediction was also employed for comparison. Both assays were used to screen 302 phenotyped accessions in a germplasm collection. The real-time PCR assay reliably discriminated all six PavMYB10.1 genotypes, and accurately predicted skin colour in all PavMYB10.1 homozygous genotypes; however, for heterozygotes, the assay was uninformative. As for the Pav-Rf-SSR assay, only two out of six observed alleles were informative, and only for mahogany market class. Using combined approach, fruit skin colour could be successfully predicted in 67.9% of all germplasm samples. The real-time PCR assay for PavMYB10.1 genotyping thus represents suitable tool for laboratories interested in MAS for skin colour in sweet cherries.

Název v anglickém jazyce

A new allelic discrimination real-time PCR assay for PavMYB10.1 genotyping to predict sweet cherry fruit colour: a comparison with the Pav-Rf-SSR assay

Popis výsledku anglicky

Fruit skin colour in cherries is one of the most important market characteristics with customer preferences varying across cultures and countries. Based on skin colour, cherries can be divided into three market classes: yellow, blush, and mahogany. The PavMYB10.1 gene has been recently shown to be a key player in fruit colouration, and three alleles (PavMYB10.1a [WT (5A)]; PavMYB10.1b [Mut(4A)]; and PavMYB10.1c [InDel]) have been associated with cherry skin colour. In the present study, an allelic discrimination real-time PCR assay was developed for marker-assisted selection (MAS). The Pav-Rf-SSR assay that had previously been described for colour prediction was also employed for comparison. Both assays were used to screen 302 phenotyped accessions in a germplasm collection. The real-time PCR assay reliably discriminated all six PavMYB10.1 genotypes, and accurately predicted skin colour in all PavMYB10.1 homozygous genotypes; however, for heterozygotes, the assay was uninformative. As for the Pav-Rf-SSR assay, only two out of six observed alleles were informative, and only for mahogany market class. Using combined approach, fruit skin colour could be successfully predicted in 67.9% of all germplasm samples. The real-time PCR assay for PavMYB10.1 genotyping thus represents suitable tool for laboratories interested in MAS for skin colour in sweet cherries.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40105 - Horticulture, viticulture

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Horticultural Science and Biotechnology

ISSN

1462-0316

e-ISSN

—

Svazek periodika

96

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

260-269

Kód UT WoS článku

000589817500001

EID výsledku v databázi Scopus

2-s2.0-85094648371