Whey substrates enriched by substances supporting nisine production

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F17%3AN0000074" target="_blank" >RIV/26722861:_____/17:N0000074 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.biomicroworld2017.org/files/bookofabstracts.pdf" target="_blank" >http://www.biomicroworld2017.org/files/bookofabstracts.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Whey substrates enriched by substances supporting nisine production

Popis výsledku v původním jazyce

Nisin is known for its antimicrobial activity against many pathogenic microorganisms. Due to this reason, it is used as a food preservative approved by World Health Organization. The aim of this study was to assemble an optimal substrate composition for maximal nisin production. Reconstituted sweet whey and demineralized whey were used as a base mediums. These base mediums were enriched by combinations of protein and carbohydrate components (2 % peptone, soya peptone or casein hydrolysate, 5 % and 10 % lactose or maltodextrin). Nisin production was monitored by agar diffusion method. Lactococcus lactis subsp. lactis CCDM 731 was selected as a nisin-producing strain and Micrococcus luteus ATCC 10240 was used as an indicator of growth inhibition. The nisin production was higher in all enriched mediums compared to the basal whey mediums. Based on our results, it is obvious that fortified demineralized whey medium seems to be more suitable substrate for nisin production than fortified sweet whey medium. Our results also suggest that fortification of basal whey substrates by combination of protein and carbohydrate supplements significantly increases nisin production. Casein hydrolysate and maltodextrin were the most suitable combination for fortification of both sweet whey and demineralized whey medium.

Název v anglickém jazyce

Whey substrates enriched by substances supporting nisine production

Popis výsledku anglicky

Nisin is known for its antimicrobial activity against many pathogenic microorganisms. Due to this reason, it is used as a food preservative approved by World Health Organization. The aim of this study was to assemble an optimal substrate composition for maximal nisin production. Reconstituted sweet whey and demineralized whey were used as a base mediums. These base mediums were enriched by combinations of protein and carbohydrate components (2 % peptone, soya peptone or casein hydrolysate, 5 % and 10 % lactose or maltodextrin). Nisin production was monitored by agar diffusion method. Lactococcus lactis subsp. lactis CCDM 731 was selected as a nisin-producing strain and Micrococcus luteus ATCC 10240 was used as an indicator of growth inhibition. The nisin production was higher in all enriched mediums compared to the basal whey mediums. Based on our results, it is obvious that fortified demineralized whey medium seems to be more suitable substrate for nisin production than fortified sweet whey medium. Our results also suggest that fortification of basal whey substrates by combination of protein and carbohydrate supplements significantly increases nisin production. Casein hydrolysate and maltodextrin were the most suitable combination for fortification of both sweet whey and demineralized whey medium.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40101 - Agriculture

Projekt

<a href="/cs/project/QJ1310254" target="_blank" >QJ1310254: Výzkum využití syrovátky, jako odpadní látky mlékárenského průmyslu, k produkci antimikrobiálních sloučenin pro modifikace hydrofilních polymerních systémů s využitím v kosmetických a medicinálních aplikacích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů