Novel identification methods including a species-specific PCR for hazardous Bacillus species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F19%3AN0000048" target="_blank" >RIV/26722861:_____/19:N0000048 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/19:43919316

Výsledek na webu

<a href="https://www.akademiai.com/doi/10.1556/066.2019.48.4.2" target="_blank" >https://www.akademiai.com/doi/10.1556/066.2019.48.4.2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1556/066.2019.48.4.2" target="_blank" >10.1556/066.2019.48.4.2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Novel identification methods including a species-specific PCR for hazardous Bacillus species

Popis výsledku v původním jazyce

Fifteen strains of Bacillus spp. (three B. cereus strains, one B. subtilis, B. macerans, B. pumilus, and B. mycoides strains, and eight unknown isolates from the food-industry) were identified on species level with three designed pairs of primers for B. cereus, B. licheniformis, and B. subtilis. Primers designed for B. licheniformis (BlichF and BlichR) and B. subtilis (BsubF and BsubR) allowed specific species identification, whereas the designed pair of primers for B. cereus (BcerF2 and BcerR2) showed specificity for B. cereus sensu lato, because both B. cereus strains and B. mycoides gave positive reaction. Bacilli identification was also carried out with routine API method with unsatisfactory results. The (GTG)5-PCR method was used for strain characterization, enabling bacilli classification into separate clusters according to their taxonomic designations.

Název v anglickém jazyce

Novel identification methods including a species-specific PCR for hazardous Bacillus species

Popis výsledku anglicky

Fifteen strains of Bacillus spp. (three B. cereus strains, one B. subtilis, B. macerans, B. pumilus, and B. mycoides strains, and eight unknown isolates from the food-industry) were identified on species level with three designed pairs of primers for B. cereus, B. licheniformis, and B. subtilis. Primers designed for B. licheniformis (BlichF and BlichR) and B. subtilis (BsubF and BsubR) allowed specific species identification, whereas the designed pair of primers for B. cereus (BcerF2 and BcerR2) showed specificity for B. cereus sensu lato, because both B. cereus strains and B. mycoides gave positive reaction. Bacilli identification was also carried out with routine API method with unsatisfactory results. The (GTG)5-PCR method was used for strain characterization, enabling bacilli classification into separate clusters according to their taxonomic designations.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40500 - Other agricultural sciences

Projekt

<a href="/cs/project/QJ1210300" target="_blank" >QJ1210300: Systémy jištění kvality a bezpečnosti mlékárenských výrobků vhodnými metodami aplikovatelnými v praxi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Alimentaria

ISSN

0139-3006

e-ISSN

1588-2535

Svazek periodika

48

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

HU - Maďarsko

Počet stran výsledku

8

Strana od-do

415 – 422

Kód UT WoS článku

000499075600002

EID výsledku v databázi Scopus

2-s2.0-85079223523