Detection and quantification of goat milk adulteration by cow milk using 1H NMR.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F19%3AN0000060" target="_blank" >RIV/26722861:_____/19:N0000060 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection and quantification of goat milk adulteration by cow milk using 1H NMR.

Popis výsledku v původním jazyce

This poster and proceedings abstract were presented on the conference „Hygiena Alimentorum XL – Safety and Quality of Dairy and Vegetable Commodities“ on 15th – 17th May 2019 in Košice, Slovak Republic. According to consumer's beliefs, goat milk is an important part of human diet, because of it is greater similarity to human milk, higher proportion of small milk fat globules for easier digestion and different allergenic properties compared with cow milk. The popularity of goat milk in the Czech Republic is on the rise, however, there is a risk associated with adulteration with cow milk, which is a more affordable and cheaper alternative. Nuclear magnetic resonance (NMR) spectroscopy is a useful tool for milk analysis. Through metabolomics, it possible to find biomarkers that are associated with the health status of animals, geographical origin, species authentication or feeding assessment. In this study, we analysed ten samples of pasteurised goat milk and ten samples of cow milk from different farms by 1H NMR-based metabolomics to detect biomarkers of contamination and adulteration. NMR spectra were acquired on a Bruker 500 MHz AVANCE III HD NMR Spectrometer, operating at 500,23 MHz. The spectrometer was equipped with a TXI z-gradient BBFO probe (5 mm) and autosampler, and controlled by TOPSPIN version 3.57. Spectra were measured using 1D NOESY pulse sequence, at the temperature 298 K, 64k data points, acquisition time 4s, relaxation delay 1s, mixing time 100 ms, spectral with 16 ppm. Metabolomics approach including PCA, OPLS-DA models and pairwise comparisons, identified ten compounds different between goat and cow milk, hence providing characteristic biomarkers for detection of adulteration. The results show that biomarkers such as hippurate, lysine, glycine, lactate and creatine are clearly associated with the goat milk. It can be concluded that 1H NMR spectroscopy has a potential in quality assessment of goat and cow milk in our geographic area. These results suggest that 1H NMR could be used for distinguishing adulteration and contamination of goat milk and milk products and potential development of a robust fraud detection method useful for inspection authorities.

Název v anglickém jazyce

Detection and quantification of goat milk adulteration by cow milk using 1H NMR.

Popis výsledku anglicky

This poster and proceedings abstract were presented on the conference „Hygiena Alimentorum XL – Safety and Quality of Dairy and Vegetable Commodities“ on 15th – 17th May 2019 in Košice, Slovak Republic. According to consumer's beliefs, goat milk is an important part of human diet, because of it is greater similarity to human milk, higher proportion of small milk fat globules for easier digestion and different allergenic properties compared with cow milk. The popularity of goat milk in the Czech Republic is on the rise, however, there is a risk associated with adulteration with cow milk, which is a more affordable and cheaper alternative. Nuclear magnetic resonance (NMR) spectroscopy is a useful tool for milk analysis. Through metabolomics, it possible to find biomarkers that are associated with the health status of animals, geographical origin, species authentication or feeding assessment. In this study, we analysed ten samples of pasteurised goat milk and ten samples of cow milk from different farms by 1H NMR-based metabolomics to detect biomarkers of contamination and adulteration. NMR spectra were acquired on a Bruker 500 MHz AVANCE III HD NMR Spectrometer, operating at 500,23 MHz. The spectrometer was equipped with a TXI z-gradient BBFO probe (5 mm) and autosampler, and controlled by TOPSPIN version 3.57. Spectra were measured using 1D NOESY pulse sequence, at the temperature 298 K, 64k data points, acquisition time 4s, relaxation delay 1s, mixing time 100 ms, spectral with 16 ppm. Metabolomics approach including PCA, OPLS-DA models and pairwise comparisons, identified ten compounds different between goat and cow milk, hence providing characteristic biomarkers for detection of adulteration. The results show that biomarkers such as hippurate, lysine, glycine, lactate and creatine are clearly associated with the goat milk. It can be concluded that 1H NMR spectroscopy has a potential in quality assessment of goat and cow milk in our geographic area. These results suggest that 1H NMR could be used for distinguishing adulteration and contamination of goat milk and milk products and potential development of a robust fraud detection method useful for inspection authorities.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1920222" target="_blank" >QK1920222: Nové spolehlivé metody detekce falšování kozího a ovčího mléka a mléčných výrobků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů