Comparison of FT-NIR spectroscopy and ELISA for detection of adulteration of goat cheeses with cow's milk

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43907972" target="_blank" >RIV/62156489:43210/16:43907972 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28110/16:43874511

Výsledek na webu

<a href="https://doi.org/10.5740/jaoacint.15-0190" target="_blank" >https://doi.org/10.5740/jaoacint.15-0190</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5740/jaoacint.15-0190" target="_blank" >10.5740/jaoacint.15-0190</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of FT-NIR spectroscopy and ELISA for detection of adulteration of goat cheeses with cow's milk

Popis výsledku v původním jazyce

This study investigated the ability of two methods to detect adulteration of goat cheeses via the addition of cow's milk, with a negligible effect on the raw materials. Cheeses were produced from a mixture of goat's and cow's milk and were then analyzed by Fourier transform near-IR (FT-NIR) spectroscopy and competitive ELISA. The cheese spectra were scanned in the spectroscope in reflectance mode on an integrating sphere at 80 scans and a resolution of 4 cmMINUS SIGN 1. The spectra were evaluated via discriminant analysis, and a calibration was created via a partial least-squares algorithm to quantify the cow's milk admixture. A correlation coefficient of R = 0.999 was reached with a standard error of calibration of 0.0407. The results were statistically processed to a median value via a t-test. Adulteration detection by the ELISA method was performed using a commercial Milk Fraud/Bovine ELISA kit. It was found that the FT-NIR spectroscopy method is capable of detecting an admixture of cow's milk in goat cheese as small as 1%. The ELISA method did not return satisfactory results for the detection of adulteration with cow's milk.

Název v anglickém jazyce

Comparison of FT-NIR spectroscopy and ELISA for detection of adulteration of goat cheeses with cow's milk

Popis výsledku anglicky

This study investigated the ability of two methods to detect adulteration of goat cheeses via the addition of cow's milk, with a negligible effect on the raw materials. Cheeses were produced from a mixture of goat's and cow's milk and were then analyzed by Fourier transform near-IR (FT-NIR) spectroscopy and competitive ELISA. The cheese spectra were scanned in the spectroscope in reflectance mode on an integrating sphere at 80 scans and a resolution of 4 cmMINUS SIGN 1. The spectra were evaluated via discriminant analysis, and a calibration was created via a partial least-squares algorithm to quantify the cow's milk admixture. A correlation coefficient of R = 0.999 was reached with a standard error of calibration of 0.0407. The results were statistically processed to a median value via a t-test. Adulteration detection by the ELISA method was performed using a commercial Milk Fraud/Bovine ELISA kit. It was found that the FT-NIR spectroscopy method is capable of detecting an admixture of cow's milk in goat cheese as small as 1%. The ELISA method did not return satisfactory results for the detection of adulteration with cow's milk.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

—

Projekt

<a href="/cs/project/QJ1230044" target="_blank" >QJ1230044: Stanovení parametrů pro legislativní hodnocení kvality a zdravotní nezávadnosti syrového mléka krav, ovcí a koz</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of AOAC International

ISSN

1060-3271

e-ISSN

—

Svazek periodika

99

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

180-186

Kód UT WoS článku

000371953400022

EID výsledku v databázi Scopus

2-s2.0-84962374828