Adhesion of EPS-producing lactic acid bacteria from breast milk and dairy sources to the Caco-2 and HT29 intestinal cell tissue models

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F24%3AN0000055" target="_blank" >RIV/26722861:_____/24:N0000055 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/24:43930767

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Adhesion of EPS-producing lactic acid bacteria from breast milk and dairy sources to the Caco-2 and HT29 intestinal cell tissue models

Popis výsledku v původním jazyce

Adhesion is one of the crucial properties for probiotics, as it directly influences their effectiveness in colonizing the gut and exerting their beneficial effects. Eight strains of lactic acid bacteria (LAB) originated from breast milk, semi-hard cheese and pickle juice were tested for their adhesion ability to the human intestinal cell model Caco-2 and HT29 (coculture in ratio 9:1). As a control, the strain Lactobacillus acidophilus LA5, known for its good adhesion ability, was used. The adhesion of the tested strains ranged from 0.16 to 6.20 % and exhibited strain-apecific characteristics. The highest adhesion was observed for Lacticaseibacillus paracasei subsp. paracasei 2023-13 (6.20%) and Lactobacillus helveticus 5.2.1B (5.19%), even surpassing the adhesion rate of control strain LA (4.88%). Lower adhesion abilities were observed for the rest of the strains tested. Understanding factors affecting adhesion variability, such as surface structures and host interactions, could inform strain selection for optimized probiotic formulations. Citation: Krausova G., Mrvikova I., Kavkova M., Horackova S., Vrchotova B., Nemeckova I., Ozluk G.: Adhesion of EPS-producing lactic acid bacteria from breast milk and dairy sources to the Caco-2 and HT29 intestinal cell tissue models. Ostatní – Poster, sborník abstraktů. Konference: 17th International Scientific Conference on Probiotics, Prebiotics, Gut Microbiota and Health – IPC2024 18. – 20. June 2024. Praha. Sborník abstraktů: Proceedings, p, 125. ISBN: 978-80-908394-8-8

Název v anglickém jazyce

Adhesion of EPS-producing lactic acid bacteria from breast milk and dairy sources to the Caco-2 and HT29 intestinal cell tissue models

Popis výsledku anglicky

Adhesion is one of the crucial properties for probiotics, as it directly influences their effectiveness in colonizing the gut and exerting their beneficial effects. Eight strains of lactic acid bacteria (LAB) originated from breast milk, semi-hard cheese and pickle juice were tested for their adhesion ability to the human intestinal cell model Caco-2 and HT29 (coculture in ratio 9:1). As a control, the strain Lactobacillus acidophilus LA5, known for its good adhesion ability, was used. The adhesion of the tested strains ranged from 0.16 to 6.20 % and exhibited strain-apecific characteristics. The highest adhesion was observed for Lacticaseibacillus paracasei subsp. paracasei 2023-13 (6.20%) and Lactobacillus helveticus 5.2.1B (5.19%), even surpassing the adhesion rate of control strain LA (4.88%). Lower adhesion abilities were observed for the rest of the strains tested. Understanding factors affecting adhesion variability, such as surface structures and host interactions, could inform strain selection for optimized probiotic formulations. Citation: Krausova G., Mrvikova I., Kavkova M., Horackova S., Vrchotova B., Nemeckova I., Ozluk G.: Adhesion of EPS-producing lactic acid bacteria from breast milk and dairy sources to the Caco-2 and HT29 intestinal cell tissue models. Ostatní – Poster, sborník abstraktů. Konference: 17th International Scientific Conference on Probiotics, Prebiotics, Gut Microbiota and Health – IPC2024 18. – 20. June 2024. Praha. Sborník abstraktů: Proceedings, p, 125. ISBN: 978-80-908394-8-8

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40101 - Agriculture

Projekt

<a href="/cs/project/QK22010186" target="_blank" >QK22010186: Postbiotika, bakteriální exopolysacharidy a nové oligosacharidy pro funkční synbiotické fermentované výrobky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů