Analysis of microbial phospholipids in processes of biomonitoring of soil condition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13420%2F19%3A43895092" target="_blank" >RIV/44555601:13420/19:43895092 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/44555601:13520/19:43895092

Výsledek na webu

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DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21285/2227-2925-2019-9-1-44-52" target="_blank" >10.21285/2227-2925-2019-9-1-44-52</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of microbial phospholipids in processes of biomonitoring of soil condition

Popis výsledku v původním jazyce

The purpose of this work was to assess the possibility of applying the method of phospholipid fatty acid analysis in the composition of lipids of cell membranes of soil microorganisms for monitoring the state of the soil in the process of recovery. The experimental part consisted in a determination of phospholipid fatty acids (FFA), including extraction of phospholipids with organic solvents, extraction of the lipid fraction using polar silicate columns, preparation of methyl esters of fatty acids by alkaline meth-anolysis and analysis of the content of FFA with gas chromatography. The identification of microorgan-isms was carried out on the basis of an evaluation of the experimental results obtained, which are repre-sented by a combination of chromatogram peaks. The impact of stress factors on microorganisms is de-termined by the presence of cis-trans-isomerism or cycles of three carbon atoms at the ends of phospho-lipid fatty acid radicals. In order to verify the objectivity of the results obtained using the proposed method for analysing microorganisms, this work also used the traditional method of controlling soil microorgan-isms based on the activity of their enzymes - glucosidases, proteases, arylsulphatases and phosphatas-es. The data obtained indicate the presence of viable soil microorganisms; however, their number does not match the control samples, indicating a failure of the recovery process. The identification results demonstrate the dominance of Gram-negative (Gr-) bacteria in the control samples in comparison with the samples in conditions of natural recovery. At the same time, in the process of natural restoration, a greater quantity of fungi was found in the soil. According to the data obtained, the largest amounts of cis - isomers and the value of cyclisation of phospholipid fatty acids are characteristic of microorganisms in the control samples, which may be due to the depletion of the substrate when using the soil for agricul-tural purposes. The experimental data obtained in the course of this work allow us to propose the proce-dure as an independent method for conducting biological monitoring of ecosystems when analysing phospholipid fatty acids in the composition of cell membrane lipids.

Název v anglickém jazyce

Analysis of microbial phospholipids in processes of biomonitoring of soil condition

Popis výsledku anglicky

The purpose of this work was to assess the possibility of applying the method of phospholipid fatty acid analysis in the composition of lipids of cell membranes of soil microorganisms for monitoring the state of the soil in the process of recovery. The experimental part consisted in a determination of phospholipid fatty acids (FFA), including extraction of phospholipids with organic solvents, extraction of the lipid fraction using polar silicate columns, preparation of methyl esters of fatty acids by alkaline meth-anolysis and analysis of the content of FFA with gas chromatography. The identification of microorgan-isms was carried out on the basis of an evaluation of the experimental results obtained, which are repre-sented by a combination of chromatogram peaks. The impact of stress factors on microorganisms is de-termined by the presence of cis-trans-isomerism or cycles of three carbon atoms at the ends of phospho-lipid fatty acid radicals. In order to verify the objectivity of the results obtained using the proposed method for analysing microorganisms, this work also used the traditional method of controlling soil microorgan-isms based on the activity of their enzymes - glucosidases, proteases, arylsulphatases and phosphatas-es. The data obtained indicate the presence of viable soil microorganisms; however, their number does not match the control samples, indicating a failure of the recovery process. The identification results demonstrate the dominance of Gram-negative (Gr-) bacteria in the control samples in comparison with the samples in conditions of natural recovery. At the same time, in the process of natural restoration, a greater quantity of fungi was found in the soil. According to the data obtained, the largest amounts of cis - isomers and the value of cyclisation of phospholipid fatty acids are characteristic of microorganisms in the control samples, which may be due to the depletion of the substrate when using the soil for agricul-tural purposes. The experimental data obtained in the course of this work allow us to propose the proce-dure as an independent method for conducting biological monitoring of ecosystems when analysing phospholipid fatty acids in the composition of cell membrane lipids.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Izvestiya Vuzov - Prikladnaya Khimiya i Biotekhnologiya

ISSN

2227-2925

e-ISSN

—

Svazek periodika

2019

Číslo periodika v rámci svazku

vol.9, no.1

Stát vydavatele periodika

RU - Ruská federace

Počet stran výsledku

9

Strana od-do

44-52

Kód UT WoS článku

000463063100006

EID výsledku v databázi Scopus

—