Determinace zdroju Bacillus cereus a Bacillus licheniformis izolovaných ze syrového a pasterovaného mléka a z jogurtů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F09%3A%230000177" target="_blank" >RIV/49608851:_____/09:#0000177 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/49608851:_____/09:#0000384

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determining the source of Bacillus cereus and Bacillus licheniformis isolated from raw milk, pasteurized milk and yoghurt

Popis výsledku v původním jazyce

Strain-specific detection of B. cereus and B. licheniformis in raw and pasteurized milk, and yoghurt during processing. Randomly selected isolates of Bacillus spp. were subjected to PCR analysis, where single primer targeting to the repetitive sequence Box elements was used to fingerprint the species. The isolates were separated into 6 different fingerprint patterns. The isolates clustered together at about the 57% similarity level with 2 main groups at the 82% and 83% similarity levels, respectively. Contamination with identical strains both of B. cereus and B. licheniformis in raw and pasteurized milk was found as well as contaminated with different strains (in the case of raw milk and yoghurt or pasteurized milk and yoghurt). Several BOX types traced in processed milk samples were not discovered in the original raw milk. Despite the limited number of strains analysed, the two Bacillus species yielded adequately detectable banding profiles, permitting differentiation of bacteria at t

Název v anglickém jazyce

Determining the source of Bacillus cereus and Bacillus licheniformis isolated from raw milk, pasteurized milk and yoghurt

Popis výsledku anglicky

Strain-specific detection of B. cereus and B. licheniformis in raw and pasteurized milk, and yoghurt during processing. Randomly selected isolates of Bacillus spp. were subjected to PCR analysis, where single primer targeting to the repetitive sequence Box elements was used to fingerprint the species. The isolates were separated into 6 different fingerprint patterns. The isolates clustered together at about the 57% similarity level with 2 main groups at the 82% and 83% similarity levels, respectively. Contamination with identical strains both of B. cereus and B. licheniformis in raw and pasteurized milk was found as well as contaminated with different strains (in the case of raw milk and yoghurt or pasteurized milk and yoghurt). Several BOX types traced in processed milk samples were not discovered in the original raw milk. Despite the limited number of strains analysed, the two Bacillus species yielded adequately detectable banding profiles, permitting differentiation of bacteria at t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QF4004" target="_blank" >QF4004: Vývoj metody pro určení původu kontaminace finálních mléčných výrobků potravními patogeny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Letters in Applied Microbiology

ISSN

0266-8254

e-ISSN

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Svazek periodika

48

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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