Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F10%3A%230000405" target="_blank" >RIV/49608851:_____/10:#0000405 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods
Popis výsledku v původním jazyce
In our study, the ornithine (speC) and lysine (cadA) decarboxylase genes were investigated in E. coli strains using the PCR methods. 26 E. coli strains isolated from raw cow, sheep and goat bulk milk samples and cheeses were evaluated. The alignment of the multiple sequences and analysis of conserved domains in the studied genes was carried out using ClustalW and BLAST programs. The conserved ODC (ornithine decarboxylase like family) domain was selected as a target for primer design of the both genes. Analysis of the sequence sources for the speC gene revealed existence of speC isosyme, featured with homology between the 66 - 98 % comparising to the speC nucleotide alignment. Also, in the case of cadA gene, we observed two groups of sequences with thehomology of approximately 60% (GenBank, cadA1, cadA2). On the basement of these facts, we designed and performed also additional PCR tests. 81 % strains were positive for speC, 75 % for speC isozyme gene, 54 % for cadA1 and 45 % for cadA2
Název v anglickém jazyce
Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods
Popis výsledku anglicky
In our study, the ornithine (speC) and lysine (cadA) decarboxylase genes were investigated in E. coli strains using the PCR methods. 26 E. coli strains isolated from raw cow, sheep and goat bulk milk samples and cheeses were evaluated. The alignment of the multiple sequences and analysis of conserved domains in the studied genes was carried out using ClustalW and BLAST programs. The conserved ODC (ornithine decarboxylase like family) domain was selected as a target for primer design of the both genes. Analysis of the sequence sources for the speC gene revealed existence of speC isosyme, featured with homology between the 66 - 98 % comparising to the speC nucleotide alignment. Also, in the case of cadA gene, we observed two groups of sequences with thehomology of approximately 60% (GenBank, cadA1, cadA2). On the basement of these facts, we designed and performed also additional PCR tests. 81 % strains were positive for speC, 75 % for speC isozyme gene, 54 % for cadA1 and 45 % for cadA2
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů