Microscope point spread function, focus and calculation of optimal microscope set-up

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F14%3A43886876" target="_blank" >RIV/60076658:12520/14:43886876 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.tandfonline.com/doi/abs/10.1080/00207160.2013.851379#.VKqbbntlw80" target="_blank" >http://www.tandfonline.com/doi/abs/10.1080/00207160.2013.851379#.VKqbbntlw80</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00207160.2013.851379" target="_blank" >10.1080/00207160.2013.851379</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microscope point spread function, focus and calculation of optimal microscope set-up

Popis výsledku v původním jazyce

If we are observing very small objects in the microscope the information appears somehow distorted. This distortion is called point spread function (PSF) and it can be measured and simulated as we show in this article. Many experiments and simulations were done in the field of confocal or deconvolution microscopy, but we want to present 3D reconstruction and measurement of PSF in the bright field (or phase contrast) microscopy. Our goal is to use this knowledge about PSF to estimate objective focus. Wewant to use this method for automatic focusing of the microscope. We measured the PSF on our microscope in bright field using a 100xobjective. We used 15 nm gold and 200 nm latex particles as point sources. We used two different simulations; first one was Airy disc simulation; the second one was Extended Nijboer-Zernike theory. Both approaches were used for description of PSF and finding the position of the focus.

Název v anglickém jazyce

Microscope point spread function, focus and calculation of optimal microscope set-up

Popis výsledku anglicky

If we are observing very small objects in the microscope the information appears somehow distorted. This distortion is called point spread function (PSF) and it can be measured and simulated as we show in this article. Many experiments and simulations were done in the field of confocal or deconvolution microscopy, but we want to present 3D reconstruction and measurement of PSF in the bright field (or phase contrast) microscopy. Our goal is to use this knowledge about PSF to estimate objective focus. Wewant to use this method for automatic focusing of the microscope. We measured the PSF on our microscope in bright field using a 100xobjective. We used 15 nm gold and 200 nm latex particles as point sources. We used two different simulations; first one was Airy disc simulation; the second one was Extended Nijboer-Zernike theory. Both approaches were used for description of PSF and finding the position of the focus.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: Jihočeské výzkumné centrum akvakultury a biodiverzity hydrocenóz</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INTERNATIONAL JOURNAL OF COMPUTER MATHEMATICS

ISSN

0020-7160

e-ISSN

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Svazek periodika

91

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

221-232

Kód UT WoS článku

000335089900006

EID výsledku v databázi Scopus

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