Genetic variants in MYF5 affected growth traits and beef quality traits in Chinese Qinchuan cattle
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900908" target="_blank" >RIV/60076658:12520/20:43900908 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.1016/j.ygeno.2020.03.018" target="_blank" >https://doi.org/10.1016/j.ygeno.2020.03.018</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ygeno.2020.03.018" target="_blank" >10.1016/j.ygeno.2020.03.018</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Genetic variants in MYF5 affected growth traits and beef quality traits in Chinese Qinchuan cattle
Popis výsledku v původním jazyce
Myogenic factor 5 plays actively roles in the regulation of myogenesis. The aims of this study are to identify the evolution information of MYF5 protein among 10 domestic and mammalian animals, to uncover the expression patterns of MYF5 gene in calves and adults of Qinchuan cattle, and to expose the genetic variants of the MYF5 gene and explore its effect on cattle growth traits and beef quality traits in Qinchuan cattle. The bioinformatics results showed that the MYF5 proteins highly conserved in different mammalian or domestic animals apart from chicken. The expression level of MYF5 gene in the heart, muscle, lung, large intestine and liver was greater than that of other tissues. PCR amplicons sequencing identified four novel SNPs at g.5738A > G, g.5785C > T and g.5816A > G in the 3rd exon region and g.6535A > G in the 3' UTR. Genotypic frequencies of g.5785C > T was harshly deviated from the HWE (P < .05). Genetic diversity was low or intermediate for the four SNPs and those SNPs were in the weak linkage disequilibrium. Association analysis results indicated g.5785C > T, g.5816A > G and g.6535A > G significant effect on growth performance and beef quality traits of Qinchuan cattle. H1H3 diplotype had greater body size and better beef quality. All the results implicate that the MYF5 gene might be applied as a promising candidate gene in Qinchuan cattle breeding.
Název v anglickém jazyce
Genetic variants in MYF5 affected growth traits and beef quality traits in Chinese Qinchuan cattle
Popis výsledku anglicky
Myogenic factor 5 plays actively roles in the regulation of myogenesis. The aims of this study are to identify the evolution information of MYF5 protein among 10 domestic and mammalian animals, to uncover the expression patterns of MYF5 gene in calves and adults of Qinchuan cattle, and to expose the genetic variants of the MYF5 gene and explore its effect on cattle growth traits and beef quality traits in Qinchuan cattle. The bioinformatics results showed that the MYF5 proteins highly conserved in different mammalian or domestic animals apart from chicken. The expression level of MYF5 gene in the heart, muscle, lung, large intestine and liver was greater than that of other tissues. PCR amplicons sequencing identified four novel SNPs at g.5738A > G, g.5785C > T and g.5816A > G in the 3rd exon region and g.6535A > G in the 3' UTR. Genotypic frequencies of g.5785C > T was harshly deviated from the HWE (P < .05). Genetic diversity was low or intermediate for the four SNPs and those SNPs were in the weak linkage disequilibrium. Association analysis results indicated g.5785C > T, g.5816A > G and g.6535A > G significant effect on growth performance and beef quality traits of Qinchuan cattle. H1H3 diplotype had greater body size and better beef quality. All the results implicate that the MYF5 gene might be applied as a promising candidate gene in Qinchuan cattle breeding.
Klasifikace
Druh
J<sub>ost</sub> - Ostatní články v recenzovaných periodicích
CEP obor
—
OECD FORD obor
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
GENOMICS
ISSN
0888-7543
e-ISSN
—
Svazek periodika
112
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
2804-2812
Kód UT WoS článku
000534479700015
EID výsledku v databázi Scopus
2-s2.0-85082526743