Identification of genetic variants the CCKAR gene and based on body measurement and carcass quality characteristics in Qinchuan beef cattle (Bos taurus)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902555" target="_blank" >RIV/60076658:12520/21:43902555 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.ejbt.2021.02.001" target="_blank" >https://doi.org/10.1016/j.ejbt.2021.02.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejbt.2021.02.001" target="_blank" >10.1016/j.ejbt.2021.02.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of genetic variants the CCKAR gene and based on body measurement and carcass quality characteristics in Qinchuan beef cattle (Bos taurus)

Popis výsledku v původním jazyce

Background: This study aimed to explore genetic polymorphisms of the CCKAR gene and their relationship with the growth and development of Qinchuan cattle which could be used as molecular markers for the improvement of the breeding of Qinchuan cattle. Results: Here, we have identified seven single nucleotide polymorphisms (SNPs) at loci g. 1463 C&gt;G; g. 1532 T&gt;A; g. 1570 G&gt;A; g. 1594 C&gt;A; g. 1640 T&gt;C; g. 1677 G&gt;C; and g. 1735 C&gt;T in the coding region of the bovine CCKAR gene. The frequencies identified on allelic and genotypic characteristics have shown that all seven SNPs diverged from the Hardy-Weinberg-Equilibrium. The SNP2, SNP3, SNP6 and SNP7 had the lowest polymorphism information content values, and remaining SNPs were found to be moderate (0.25 &lt; PIC &lt; 0.50). The genotype CG in SNP1 at loci g.1463 C&gt;G had the greatest association with WH, HW, CD and CCF, while the genotype TA at the very same loci was associated with BFT, ULA and IMF content in Qinchuan cattle. The CCKAR gene expression level in adipose tissue, small intestine, liver and skeleton muscle was found to be higher, whereas, the expression level of mRNA in organs of other digestive system including reticulum, abomasum and omasum was moderate. Some expression of CCKAR mRNA was found in the large intestine, kidney and rumen. Conclusion: In summary, our finding suggested that the CCKAR gene could be used as a potential candidate for the improvement of carcass quality and body measurements of Qinchuan cattle. How to cite: Nurgulsim K, Raza SHA, Khan R, et al. Identification of genetic variants the CCKAR gene and based on body measurement and carcass quality characteristics in Qinchuan beef cattle (Bos taurus). Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.02.001 © 2021

Název v anglickém jazyce

Identification of genetic variants the CCKAR gene and based on body measurement and carcass quality characteristics in Qinchuan beef cattle (Bos taurus)

Popis výsledku anglicky

Background: This study aimed to explore genetic polymorphisms of the CCKAR gene and their relationship with the growth and development of Qinchuan cattle which could be used as molecular markers for the improvement of the breeding of Qinchuan cattle. Results: Here, we have identified seven single nucleotide polymorphisms (SNPs) at loci g. 1463 C&gt;G; g. 1532 T&gt;A; g. 1570 G&gt;A; g. 1594 C&gt;A; g. 1640 T&gt;C; g. 1677 G&gt;C; and g. 1735 C&gt;T in the coding region of the bovine CCKAR gene. The frequencies identified on allelic and genotypic characteristics have shown that all seven SNPs diverged from the Hardy-Weinberg-Equilibrium. The SNP2, SNP3, SNP6 and SNP7 had the lowest polymorphism information content values, and remaining SNPs were found to be moderate (0.25 &lt; PIC &lt; 0.50). The genotype CG in SNP1 at loci g.1463 C&gt;G had the greatest association with WH, HW, CD and CCF, while the genotype TA at the very same loci was associated with BFT, ULA and IMF content in Qinchuan cattle. The CCKAR gene expression level in adipose tissue, small intestine, liver and skeleton muscle was found to be higher, whereas, the expression level of mRNA in organs of other digestive system including reticulum, abomasum and omasum was moderate. Some expression of CCKAR mRNA was found in the large intestine, kidney and rumen. Conclusion: In summary, our finding suggested that the CCKAR gene could be used as a potential candidate for the improvement of carcass quality and body measurements of Qinchuan cattle. How to cite: Nurgulsim K, Raza SHA, Khan R, et al. Identification of genetic variants the CCKAR gene and based on body measurement and carcass quality characteristics in Qinchuan beef cattle (Bos taurus). Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.02.001 © 2021

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electronic Journal of Biotechnology

ISSN

0717-3458

e-ISSN

—

Svazek periodika

51

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

CL - Chilská republika

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

000652394100001

EID výsledku v databázi Scopus

2-s2.0-85103084530