Ochratoxin A in roasted coffee marketed in Czech Republic: determination by liquid chromatography and fluorescence detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F23%3AN0000010" target="_blank" >RIV/60193697:_____/23:N0000010 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/23:43923567 RIV/60461373:22330/23:43926983

Výsledek na webu

<a href="https://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=62&article=2313" target="_blank" >https://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=62&article=2313</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Ochratoxin A in roasted coffee marketed in Czech Republic: determination by liquid chromatography and fluorescence detection

Popis výsledku v původním jazyce

This study dealt with determination of ochratoxin A (OTA) in roasted coffee by ultra-performance liquid chromatography and fluorescence detection (UPLC-FLD). Coffee features characteristic sensory properties, contains a wide range of beneficial substances and is among the most sold commodities worldwide. UPLC-FLD method for determination of OTA in ground roasted coffee was optimized and validated. A calibration curve of good linearity (R-2 > 0.999) was obtained for the range of 0.3-10 ng.ml(-1), and the limit of detection and limit of quantification for OTA were 0.1 mu g.kg(-1) and 0.3 mu g center dot kg(-1), respectively. The recovery of certified reference material for OTA ranged between 81.3-112.8 % and the relative standard deviation under repeatibility conditions was 11.7 %. The method was applied to the analysis of OTA in coffee obtained from retail stores in Czech Republic. Sixteen samples (29 %) of roasted coffee were positive, containing OTA in the range from 0.31 mu g center dot kg(-1) to 1.06 mu g center dot kg(-1). None of the samples analysed exceeded the legislative limit according to EU Commission Regulation No. 1881/2006.

Název v anglickém jazyce

Ochratoxin A in roasted coffee marketed in Czech Republic: determination by liquid chromatography and fluorescence detection

Popis výsledku anglicky

This study dealt with determination of ochratoxin A (OTA) in roasted coffee by ultra-performance liquid chromatography and fluorescence detection (UPLC-FLD). Coffee features characteristic sensory properties, contains a wide range of beneficial substances and is among the most sold commodities worldwide. UPLC-FLD method for determination of OTA in ground roasted coffee was optimized and validated. A calibration curve of good linearity (R-2 > 0.999) was obtained for the range of 0.3-10 ng.ml(-1), and the limit of detection and limit of quantification for OTA were 0.1 mu g.kg(-1) and 0.3 mu g center dot kg(-1), respectively. The recovery of certified reference material for OTA ranged between 81.3-112.8 % and the relative standard deviation under repeatibility conditions was 11.7 %. The method was applied to the analysis of OTA in coffee obtained from retail stores in Czech Republic. Sixteen samples (29 %) of roasted coffee were positive, containing OTA in the range from 0.31 mu g center dot kg(-1) to 1.06 mu g center dot kg(-1). None of the samples analysed exceeded the legislative limit according to EU Commission Regulation No. 1881/2006.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food and Nutrition Research

ISSN

1336-8672

e-ISSN

1338-4260

Svazek periodika

62

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

7

Strana od-do

191-197

Kód UT WoS článku

001018898300011

EID výsledku v databázi Scopus

2-s2.0-85161842010