Occurrence of ochratoxin A and its stereoisomeric degradation product in various types of coffee available in the Czech market

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43921033" target="_blank" >RIV/60461373:22330/20:43921033 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.wageningenacademic.com/doi/10.3920/WMJ2019.2507" target="_blank" >https://www.wageningenacademic.com/doi/10.3920/WMJ2019.2507</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3920/WMJ2019.2507" target="_blank" >10.3920/WMJ2019.2507</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence of ochratoxin A and its stereoisomeric degradation product in various types of coffee available in the Czech market

Popis výsledku v původním jazyce

Ochratoxin A (OTA) belongs among the most frequently occurring mycotoxins in coffee. In order to investigate its contamination levels in products currently available in the market, a broad set of coffee samples (103 in total) collected between 2016 and 2018 in the Czech Republic was investigated. Aqueous-methanolic extracts purified by using immunoaffinity columns were analysed by ultra-performance liquid chromatography coupled with tandem mass spectrometry (U-HPLC-MS/MS). The undertaken study revealed a relatively low OTA contamination of roasted coffee (in the range 0.2-2.5 µg/kg with the mean concentration of 0.6 µg/kg, and 71% of positive samples). The roasted coffee samples did not exceed the maximum limit of 5 µg/kg set by 1881/2006/EC. With regard to instant coffee samples, OTA concentrations were considerably higher. All the samples were positive, with a mean concentration of 2.9 µg/kg (ranging from 0.6 to 12.8 µg/kg, with 100% of positive samples). One of the analysed samples of instant coffee even exceeded the maximum limit of 10 µg/kg (1881/2006/EC). The study further revealed a relatively high incidence of 14-(R)-OTA, stereoisomer of OTA (14-(S)-OTA), originating as its main degradation product. Its identity was confirmed by high resolution mass spectrometry (HRMS/MS). Most of the samples positive for OTA were also positive for this diastereoisomer, with signal intensities of approx. one-third to one half of the signal of 14-(S)-OTA.

Název v anglickém jazyce

Occurrence of ochratoxin A and its stereoisomeric degradation product in various types of coffee available in the Czech market

Popis výsledku anglicky

Ochratoxin A (OTA) belongs among the most frequently occurring mycotoxins in coffee. In order to investigate its contamination levels in products currently available in the market, a broad set of coffee samples (103 in total) collected between 2016 and 2018 in the Czech Republic was investigated. Aqueous-methanolic extracts purified by using immunoaffinity columns were analysed by ultra-performance liquid chromatography coupled with tandem mass spectrometry (U-HPLC-MS/MS). The undertaken study revealed a relatively low OTA contamination of roasted coffee (in the range 0.2-2.5 µg/kg with the mean concentration of 0.6 µg/kg, and 71% of positive samples). The roasted coffee samples did not exceed the maximum limit of 5 µg/kg set by 1881/2006/EC. With regard to instant coffee samples, OTA concentrations were considerably higher. All the samples were positive, with a mean concentration of 2.9 µg/kg (ranging from 0.6 to 12.8 µg/kg, with 100% of positive samples). One of the analysed samples of instant coffee even exceeded the maximum limit of 10 µg/kg (1881/2006/EC). The study further revealed a relatively high incidence of 14-(R)-OTA, stereoisomer of OTA (14-(S)-OTA), originating as its main degradation product. Its identity was confirmed by high resolution mass spectrometry (HRMS/MS). Most of the samples positive for OTA were also positive for this diastereoisomer, with signal intensities of approx. one-third to one half of the signal of 14-(S)-OTA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

WORLD MYCOTOXIN JOURNAL

ISSN

1875-0710

e-ISSN

1875-0796

Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

97-107

Kód UT WoS článku

000512312600009

EID výsledku v databázi Scopus

2-s2.0-85079236175