Determination of mycotoxins in biofilms of filamentous fungi (poster)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F24%3AN0000045" target="_blank" >RIV/60193697:_____/24:N0000045 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of mycotoxins in biofilms of filamentous fungi (poster)

Popis výsledku v původním jazyce

The occurrence of contaminating microorganisms in the food industry and the risk of food contamination depends on complex hygienic standards of the food-processing plants, but when contamination occurs, the source of the problem is often biofilm related. Mycotoxins are secondary metabolites produced by filamentous fungi such as Alternaria, Aspergillus, Fusarium and Penicillium sp. These substances represent a serious risk to human and animal health, when contained in food or animal feed. The maximum mycotoxin content is regulated by Commission Regulation (EU) 2023/915 in the European Union for aflatoxins, ochratoxin A, patulin, deoxynivalenol, zearalenone, fumonisins and citrinin. In the USA, the mycotoxin levels are regulated by the Food and the Drug Administration (FDA) for the same mycotoxins except zearalenone and citrinin. Pythium oligandrum is a soil-born oomycete which can be used for suppression of filamentous fungi growth due to its antagonistic and parasitic activity. It has attracted attention due to being non-pathogenic to plants but being capable of acting against pathogens to directly and indirectly protect plants from fungal infections. However, there is no information on the interaction of Pythium oligandrum with fungal biofilms. Cultures of Aspergillus niger were inoculated in 28 wells on microtiter plate with potato dextrose broth as a cultivation medium. During the cultivation spores of Pythium spp. were added into some of the wells at 24 and 48 hours of cultivation. After the cultivation, QuEChERS method was used for the extraction of mycotoxins from the whole microtiter well, from cultivation media and from the biofilm of the filamentous fungi. UPLC with mass spectrometer was used for determination of 28 mycotoxins from which 3 were found (ochratoxin A, ochratoxin B and fumonisin B2). The measured amounts were compared to the control cultivation. The amount of ochratoxin A and ochratoxin B in Pythium treated samples were 0.8 - 1.2 and 0.9 - 2.1 times higher, respectively.However, the amounts of fumonisin B2 were on average 12.1 times higher in the treated samples. This shows that using Pythium spp. as a control agent to suppress the growth of A. niger leads into Aspergillus to produce more fumonisin B2 as a defence mechanism. The poster was presented at the ACS Spring 2024 conference in New Orleans, USA which took place betwen 17th and 22nd March 2024.

Název v anglickém jazyce

Determination of mycotoxins in biofilms of filamentous fungi (poster)

Popis výsledku anglicky

The occurrence of contaminating microorganisms in the food industry and the risk of food contamination depends on complex hygienic standards of the food-processing plants, but when contamination occurs, the source of the problem is often biofilm related. Mycotoxins are secondary metabolites produced by filamentous fungi such as Alternaria, Aspergillus, Fusarium and Penicillium sp. These substances represent a serious risk to human and animal health, when contained in food or animal feed. The maximum mycotoxin content is regulated by Commission Regulation (EU) 2023/915 in the European Union for aflatoxins, ochratoxin A, patulin, deoxynivalenol, zearalenone, fumonisins and citrinin. In the USA, the mycotoxin levels are regulated by the Food and the Drug Administration (FDA) for the same mycotoxins except zearalenone and citrinin. Pythium oligandrum is a soil-born oomycete which can be used for suppression of filamentous fungi growth due to its antagonistic and parasitic activity. It has attracted attention due to being non-pathogenic to plants but being capable of acting against pathogens to directly and indirectly protect plants from fungal infections. However, there is no information on the interaction of Pythium oligandrum with fungal biofilms. Cultures of Aspergillus niger were inoculated in 28 wells on microtiter plate with potato dextrose broth as a cultivation medium. During the cultivation spores of Pythium spp. were added into some of the wells at 24 and 48 hours of cultivation. After the cultivation, QuEChERS method was used for the extraction of mycotoxins from the whole microtiter well, from cultivation media and from the biofilm of the filamentous fungi. UPLC with mass spectrometer was used for determination of 28 mycotoxins from which 3 were found (ochratoxin A, ochratoxin B and fumonisin B2). The measured amounts were compared to the control cultivation. The amount of ochratoxin A and ochratoxin B in Pythium treated samples were 0.8 - 1.2 and 0.9 - 2.1 times higher, respectively.However, the amounts of fumonisin B2 were on average 12.1 times higher in the treated samples. This shows that using Pythium spp. as a control agent to suppress the growth of A. niger leads into Aspergillus to produce more fumonisin B2 as a defence mechanism. The poster was presented at the ACS Spring 2024 conference in New Orleans, USA which took place betwen 17th and 22nd March 2024.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů