Dilute and Shoot: A novel approach for determination of chlormequat and mepiquat in beer(poster)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F25%3AN0000043" target="_blank" >RIV/60193697:_____/25:N0000043 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dilute and Shoot: A novel approach for determination of chlormequat and mepiquat in beer(poster)

Popis výsledku v původním jazyce

The extensive use of pesticides in agriculture has led to the presence of their residues in soil, water sources, and crops. Among these, the herbicides chlormequat and mepiquat are commonly employed to regulate plant growth. Both function by inhibiting gibberellin biosynthesis: chlormequat limits cell elongation, resulting in shorter, sturdier stems, while also influencing the plant’s developmental cycle, promoting flowering and increasing yield. Mepiquat exhibits similar effects due to its comparable mode of action. The European Union has established maximum residue limits (MRLs) in barley at 7 mg/kg for chlormequat and 4 mg/kg for mepiquat. However, the extent to which these compounds are transferred from raw brewing materials into beer remains poorly understood, and no regulatory limits currently exist for their concentrations in beer. This study aimed to develop and validate a rapid, cost-effective, and straightforward analytical method for the simultaneous determination of chlormequat and mepiquat in beer. A Dilute and Shoot strategy was evaluated: degassed beer samples were diluted with acetonitrile and analysed using a Waters Acquity UPLC system coupled with a XEVO TQ-S micro mass spectrometer with electrospray ionization (ESI). Chromatographic separation was achieved using an isocratic elution on a Kinetex® 1.7 μm (100 × 2.1 mm) HILIC column. Method performance was assessed in terms of linearity, detection and quantification limits, precision, and accuracy. Recoveries were 89% for chlormequat and 84% for mepiquat, with relative standard deviations (RSDs) not exceeding 4%. A total of 86 beer samples were analysed, revealing contamination in 77% of samples, with concentrations ranging from 0.1 to 45.3 μg/L. The data were presented via poster at 10th School of Liquid Chromatography in Zaječí on 18th to 21st of May 2025.

Název v anglickém jazyce

Dilute and Shoot: A novel approach for determination of chlormequat and mepiquat in beer(poster)

Popis výsledku anglicky

The extensive use of pesticides in agriculture has led to the presence of their residues in soil, water sources, and crops. Among these, the herbicides chlormequat and mepiquat are commonly employed to regulate plant growth. Both function by inhibiting gibberellin biosynthesis: chlormequat limits cell elongation, resulting in shorter, sturdier stems, while also influencing the plant’s developmental cycle, promoting flowering and increasing yield. Mepiquat exhibits similar effects due to its comparable mode of action. The European Union has established maximum residue limits (MRLs) in barley at 7 mg/kg for chlormequat and 4 mg/kg for mepiquat. However, the extent to which these compounds are transferred from raw brewing materials into beer remains poorly understood, and no regulatory limits currently exist for their concentrations in beer. This study aimed to develop and validate a rapid, cost-effective, and straightforward analytical method for the simultaneous determination of chlormequat and mepiquat in beer. A Dilute and Shoot strategy was evaluated: degassed beer samples were diluted with acetonitrile and analysed using a Waters Acquity UPLC system coupled with a XEVO TQ-S micro mass spectrometer with electrospray ionization (ESI). Chromatographic separation was achieved using an isocratic elution on a Kinetex® 1.7 μm (100 × 2.1 mm) HILIC column. Method performance was assessed in terms of linearity, detection and quantification limits, precision, and accuracy. Recoveries were 89% for chlormequat and 84% for mepiquat, with relative standard deviations (RSDs) not exceeding 4%. A total of 86 beer samples were analysed, revealing contamination in 77% of samples, with concentrations ranging from 0.1 to 45.3 μg/L. The data were presented via poster at 10th School of Liquid Chromatography in Zaječí on 18th to 21st of May 2025.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40500 - Other agricultural sciences

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů