Stanovení počtu a isolace a identifikace bifidobakterií z kojeneckých výkalů.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7069" target="_blank" >RIV/60460709:41210/04:7069 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enumeration, isolation and identification of bifidobacteria from infant feces.

Popis výsledku v původním jazyce

The aim of our work was to enumerate, isolate and identify bifidobacteria in infant faeces. Thirty three fully breast-fed infants in the age between 1 and 12 weeks were investigated. FISH kit for the quantitative detection of Bifidobacterium sp. was compared with culture method. Bifidobacteria in faecal samples were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/L) and glacial acetic acid (1 ml/L). Pure cultures were identified to the genus level using fructose-6-phosphate phosphoketolase test, and to the species level using API 50 CHL and API ID 32 A Rapid tests followed by computer program Bacter. The identification to the species level was also carried out by PCR method using species-specific primers. Bifidobacteria counts in most faecal samples determined by both TPY agar and FISH procedure were from 108 to 1011 CFU/g. Three infants did not contain any bifidobacteria in their faecal samples. One of them was delivered by caesarean section and tw

Název v anglickém jazyce

Enumeration, isolation and identification of bifidobacteria from infant feces.

Popis výsledku anglicky

The aim of our work was to enumerate, isolate and identify bifidobacteria in infant faeces. Thirty three fully breast-fed infants in the age between 1 and 12 weeks were investigated. FISH kit for the quantitative detection of Bifidobacterium sp. was compared with culture method. Bifidobacteria in faecal samples were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/L) and glacial acetic acid (1 ml/L). Pure cultures were identified to the genus level using fructose-6-phosphate phosphoketolase test, and to the species level using API 50 CHL and API ID 32 A Rapid tests followed by computer program Bacter. The identification to the species level was also carried out by PCR method using species-specific primers. Bifidobacteria counts in most faecal samples determined by both TPY agar and FISH procedure were from 108 to 1011 CFU/g. Three infants did not contain any bifidobacteria in their faecal samples. One of them was delivered by caesarean section and tw

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Svazek periodika

2

Číslo periodika v rámci svazku

49

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

209-212

Kód UT WoS článku

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EID výsledku v databázi Scopus

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