Počty, isolace a identifikace bifidobakterií izolovaných z mléčných výrobků.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7932" target="_blank" >RIV/60460709:41210/04:7932 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enumeration, isolation, and identification of bifidobacteria from dairy products.

Popis výsledku v původním jazyce

Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/l). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) andby the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46°C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification wasalso carried out by PCR method using genus- and species-specific primers. The bifidobacterial counts in products tested varied from 2.37 to 7.17 log CFU/ml. High selectivity was seen for modified TPY agar from which all isolates were identified as bifidobacteria. While most strains were identified as B. animalis using Bacter program, the same isolates had positive reaction with B. lactis-specific primers. Strain isolated from Rajo yogurt was identified as B. longum by Bacter as well as

Název v anglickém jazyce

Enumeration, isolation, and identification of bifidobacteria from dairy products.

Popis výsledku anglicky

Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/l). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) andby the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46°C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification wasalso carried out by PCR method using genus- and species-specific primers. The bifidobacterial counts in products tested varied from 2.37 to 7.17 log CFU/ml. High selectivity was seen for modified TPY agar from which all isolates were identified as bifidobacteria. While most strains were identified as B. animalis using Bacter program, the same isolates had positive reaction with B. lactis-specific primers. Strain isolated from Rajo yogurt was identified as B. longum by Bacter as well as

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Agriculturae Slovenica

ISSN

1581-9175

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

84

Stát vydavatele periodika

SI - Slovinská republika

Počet stran výsledku

6

Strana od-do

31-36

Kód UT WoS článku

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EID výsledku v databázi Scopus

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