Příprava galaktooligosacharidů v membránovém reaktoru

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00021225" target="_blank" >RIV/60461373:22330/08:00021225 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of galacto-oligosaccharides using membrane reactor

Popis výsledku v původním jazyce

Galacto-oligosaccharides have many biological functions, e.g. selective improvement of growth of beneficial bifidobacteria, improved absorption of calcium and magnesium, and improved detoxification of the body. Galacto - oligosaccharides are produced from lactose by a glycosyl transfer of one or more D-galactosyl units onto the D-galactose moiety of lactose catalysed by ?-galactosidase. Many factors have influence on this reaction e.g. temperature, origin of enzyme, concentration of substrate. This reaction can be carried out in batch and continuous system. We used membrane reactor with ultrafiltration ceramic membrane (150 KDa). Lactose in phosphate buffer (200 g.L-1), recombined whey (197 g.L-1) and ultrafiltration permeate (320 g.L-1) were used as substrate. Concentration of used enzyme Maxilact LX 5000 was 2 g.L-1. Temperature during reaction was 37 °C. Reaction product were analysed by HPLC with cation exchange column, demineralised water was used as the mobile phase. The highest

Název v anglickém jazyce

Preparation of galacto-oligosaccharides using membrane reactor

Popis výsledku anglicky

Galacto-oligosaccharides have many biological functions, e.g. selective improvement of growth of beneficial bifidobacteria, improved absorption of calcium and magnesium, and improved detoxification of the body. Galacto - oligosaccharides are produced from lactose by a glycosyl transfer of one or more D-galactosyl units onto the D-galactose moiety of lactose catalysed by ?-galactosidase. Many factors have influence on this reaction e.g. temperature, origin of enzyme, concentration of substrate. This reaction can be carried out in batch and continuous system. We used membrane reactor with ultrafiltration ceramic membrane (150 KDa). Lactose in phosphate buffer (200 g.L-1), recombined whey (197 g.L-1) and ultrafiltration permeate (320 g.L-1) were used as substrate. Concentration of used enzyme Maxilact LX 5000 was 2 g.L-1. Temperature during reaction was 37 °C. Reaction product were analysed by HPLC with cation exchange column, demineralised water was used as the mobile phase. The highest

Druh

D - Stať ve sborníku

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CD-ROM of Full Texts CHISA 2008

ISBN

978-80-02-02047-9

ISSN

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e-ISSN

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Počet stran výsledku

8

Strana od-do

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Název nakladatele

Process Engineering Publisher

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

24. 8. 2008

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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