Preparation of the galactooligosaccharides in the batch reactor: Comparison of a commercial ?-galactosidase and crude cell extracts from lactic acid bacteria

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of the galactooligosaccharides in the batch reactor: Comparison of a commercial ?-galactosidase and crude cell extracts from lactic acid bacteria

Popis výsledku v původním jazyce

Galactooligosaccharides are produced from lactose by a glycosyl transfer of one or more D galactosyl units onto the D-galactose moiety of lactose catalysed by ?-galactosidase. Many factors have influence on this reaction e.g. temperature, concentration of substrate and origin of enzyme. Enzymes from different sources produce different types of galactooligosaccharides. We used three different type of enzyme in this work. The one of them was from commercial source and the other two were isolated from bacteria in form of crude cell extract. Lactobacillus delbruckii ssp. bulgaricus and Streptococcus thermophilus were used as bacteria sources and Maxilact LX 5000 was used as commercial source. Lactose in buffer, ultrafiltration permeate and recombined wheywere used as substrates. The concentration of enzymes was 0.5 U.mL-1 and concentration of used substrates was 50 g.L-1. The highest yield was obtained for both crude cell extracts but Lactobacillus delbruckii ssp. bulgaricus had lower yie

Název v anglickém jazyce

Preparation of the galactooligosaccharides in the batch reactor: Comparison of a commercial ?-galactosidase and crude cell extracts from lactic acid bacteria

Popis výsledku anglicky

Galactooligosaccharides are produced from lactose by a glycosyl transfer of one or more D galactosyl units onto the D-galactose moiety of lactose catalysed by ?-galactosidase. Many factors have influence on this reaction e.g. temperature, concentration of substrate and origin of enzyme. Enzymes from different sources produce different types of galactooligosaccharides. We used three different type of enzyme in this work. The one of them was from commercial source and the other two were isolated from bacteria in form of crude cell extract. Lactobacillus delbruckii ssp. bulgaricus and Streptococcus thermophilus were used as bacteria sources and Maxilact LX 5000 was used as commercial source. Lactose in buffer, ultrafiltration permeate and recombined wheywere used as substrates. The concentration of enzymes was 0.5 U.mL-1 and concentration of used substrates was 50 g.L-1. The highest yield was obtained for both crude cell extracts but Lactobacillus delbruckii ssp. bulgaricus had lower yie

Druh

D - Stať ve sborníku

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CHISA 2010

ISBN

978-80-02-02210-7

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

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Název nakladatele

Process Engineering Publisher, Novosad, J.

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

1. 1. 2010

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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