Enzymatic synthesis of galactooligasaccharides using commercial β-galactosidases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919914" target="_blank" >RIV/60461373:22330/19:43919914 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/19:43919916

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Enzymatic synthesis of galactooligasaccharides using commercial β-galactosidases

Popis výsledku v původním jazyce

Galactooligosaccharides (GOS) are widely used in the functional food industry due health-promoting effects as a prebiotic in humans. GOS can be synthesized by transgalactosylation reactions from lactose using β-galactosidase. In this study we investigate enzymatic activity on GOS formation of the next commercial enzymes: Maxilact LGI 5000, Maxilact A4 MG and two new enzymes - NOLA Fit 5500 and Saphera 2600 L. The aim of the present work was to gain the highest yield of galactooligosaccharides using commercial β-galactosidases and reduced concentration of residual lactose. The experiments were held at 40°C with initial lactose concentration of 500-550 g∙L-1 in phosphate buffer. Qualitative and quantitative data were acquired using HPLC and HPAEC-PAD. High concentration of GOS with low lactose content in solution increases the efficiency of galactooligosaccharides purification that could be potentially used in the further investigations.

Název v anglickém jazyce

Enzymatic synthesis of galactooligasaccharides using commercial β-galactosidases

Popis výsledku anglicky

Galactooligosaccharides (GOS) are widely used in the functional food industry due health-promoting effects as a prebiotic in humans. GOS can be synthesized by transgalactosylation reactions from lactose using β-galactosidase. In this study we investigate enzymatic activity on GOS formation of the next commercial enzymes: Maxilact LGI 5000, Maxilact A4 MG and two new enzymes - NOLA Fit 5500 and Saphera 2600 L. The aim of the present work was to gain the highest yield of galactooligosaccharides using commercial β-galactosidases and reduced concentration of residual lactose. The experiments were held at 40°C with initial lactose concentration of 500-550 g∙L-1 in phosphate buffer. Qualitative and quantitative data were acquired using HPLC and HPAEC-PAD. High concentration of GOS with low lactose content in solution increases the efficiency of galactooligosaccharides purification that could be potentially used in the further investigations.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

<a href="/cs/project/QK1910024" target="_blank" >QK1910024: Využití enzymatického a probiotického potenciálu mikroorganismů k vývoji nových a zvýšení kvality a trvanlivosti stávajících mléčných a pekárenských výrobků.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů